摘要
为研究能够同时检测PEDV、TGEV、PoRV三种猪病毒性腹泻病原的方法,分别设计三套特异性的引物和探针,建立了基于Taqman探针的三重Real-Time RT-PCR检测方法,实验结果表明,该方法特异性好,灵敏度高,检测最低浓度为10 copies/μL数量级.应用该方法对广东11个地市的44份猪腹泻样品进行检测,其中PEDV阳性率为100%,TGEV、PoRV阳性率为0,证明PEDV是引起广东地区猪病毒性腹泻的重要病原.
detect the porcine epidemic diarrhea virus(PEDV),transmissible gastroenteritis virus(TGEV)and porcine rotavirus(PoRV)simultaneously,three sets of specific primers and probes were designed and a triple real-time reverse transcription-polymerase chain reaction method based on TaqMan^TM probes was established. This method had good specificity and high sensitivity,and the minimum concentration was 10 copies/μL. Forty-four samples of porcine diarrhea were detected in 11 cities of Guangdong Province,China,with this method. The positive rate of PEDV was 100%,while both TGEV and PoRV were not detected. These data demonstrated that PEDV is an important pathogen causing porcine viral diarrhea in Guangdong Province.
作者
李儒曙
苏惠龙
蒋郁明
邓湘辉
黄铮
赵福振
罗宝正
LI Rushu;SU Huilong;JIANG Yuming;DENG Xianghui;HUANG Zheng;ZHAO Fuzhen;LUO Baozheng(Zhuhai Agricultural and Rural Bureau, Zhuhai 519001 ,China;Zhuhai Animal Heaith Supervision Insitute, Zhuhai 519001 ,China;Technology Center of Gonghei Customs , 519015 ,China)
出处
《广东畜牧兽医科技》
2019年第5期41-44,52,共4页
Guangdong Journal of Animal and Veterinary Science
