摘要
目的测定牛肺表面提取物质中三氯甲烷的残留量。方法采用顶空进样毛细管气相色谱法,色谱柱为DB-624毛细管柱,载气为氮气,用ECD检测器测定牛肺表面提取物质中的三氯甲烷残留量(外标法计算)。结果分离度符合要求,质量浓度在考察范围内与峰面积的线性关系良好,r=0. 9999,平均回收率为99. 56%~100. 75%,精密度的RSD值为1. 21%,定量限为6×10-3ng·m L-1。结论所用方法准确可靠,可用于样品中三氯甲烷残留量的测定。
OBJECTIVE To determine the residual trichloromethane in calf pulmonary surfactant extracts( CPSE) by Headspace GC-ECD. METHODS The residual trichloromethane was quantitatively determined by headspace GC on DB-624 column. and the detector temperature of electron capture detector( ECD) adopted external standard method. RESULTS The peaks of residual organic solvents were completely separated. There was a good linearity in the experimental concentration( r =0. 9999). The average recovery rate ranged from 99. 56% to 100. 75%. The detection limit was 6 × 10-3 ng·m L-1. RSD of precision was less than 1. 21%. CONCLUSION The method is accurate. It can be used for the determination of residual trichloromethane in CPSE.
作者
赵璇
李群
白亦昊
周长明
ZHAO Xuan;LI Qun;BAI Yihao;ZHOU Changming(Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine,NMPA Key Laboratory for Research and Evaluation of Generic Drugs,Beijing Institute for Drug Control,Beijing 102206 P.R. China)
出处
《华西药学杂志》
CAS
CSCD
2019年第5期502-504,共3页
West China Journal of Pharmaceutical Sciences
关键词
顶空气相色谱法
电子捕获检测器
牛肺表面提取物质
三氯甲烷
残留量
外标法
程序升温
质量控制
Headspace GC
Electronic capture detector
Calf pulmonary surfactant extracts
Trichloromethane
Residual quantity
External standard method
Temperature programmed
Quality control
