摘要
目的观察p62在早期急性脊髓损伤(SCI)模型大鼠脊髓组织中的表达变化,探讨p62在急性SCI的作用。方法将SD大鼠随机分为假手术组和造模后1、3、7、14d组,每组6只。采用高空重物坠落击打方法造成大鼠SCI。采用Basso、Beattie、Bresnahan(BBB)评分法评估大鼠神经功能,取损伤的脊髓组织进行HE染色,观察病理学改变。通过实时荧光定量PCR、蛋白质印迹法和免疫组织化学方法检测急性SCI后大鼠脊髓组织中p62的表达变化。结果造模后1、3、7、14d组BBB评分分别为(2.00±0.89)分、(4.67±1.03)分、(7.83±0.75)分、(14.50±1.05)分,均低于假手术组(21.00分),差异有统计学意义(P<0.05)。造模后1、3、7、14d组损伤的脊髓组织神经元和白质髓鞘发生肿胀,随时间的延长逐渐出现变性和坏死。造模后1、3、7、14d组p62表达逐渐增加,并呈持续增长趋势。结论p62在大鼠发生急性SCI后表达持续增加,提示p62在急性SCI后发挥一定作用。
Objective To observe the expression of p62 in spinal cord of rats with early acute spinal cord injury(SCI) for exploring the role of p62 in acute SCI.Methods SD rats were randomly divided into sham group and SCI 1,3,7 and 14 d groups,with 6 rats in each group.The homemade high-altitude heavy object hitting method was used to establish the acute SCI model.Basso,Beattie,Bresnahan(BBB) scale was used to estimate the neurological function,and HE staining was used to assessed the pathological changes of spine cord tissue of rats in each group.p62 expression was detected by quantitative real-time PCR,Western blotting and immunohistochemistry.Results The BBS scores of SCI 1,3,7 and 14 d groups were 2.00±0.89,4.67±1.03,7.83±0.75 and 14.50±1.05,respectively,which were lower than sham group (BBB score 21.00) with significantly difference(P < 0.05).Local swelling was observed in the damaged neurons and glial cells of spinal cord tissues of rats in SCI 1,3,7 and 14 d groups,and degeneration and necrosis gradually appeared with the prolongation of injury time.p62 expression increased gradually in SCI 1,3,7 and 14 d groups with a continuous growth trend.Conclusion The expression of p62 is increased after acute SCI in rats,suggesting that p62 play a certain role in acute SCI.
作者
沈宝良
李云飞
韩擎天
高生
陈智
沈洪兴
殷勇
SHEN Bao-liang;LI Yun-fei;HAN Qing-tian;GAO Sheng;CHEN Zhi;SHEN Hong-xing(Department of Orthopaedics,Central Hospital of Jiading District,Shanghai University of Medicine and Health Sciences,Shanghai 201800,China;Department of Orthopaedics,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200001,China)
出处
《脊柱外科杂志》
2019年第5期350-354,共5页
Journal of Spinal Surgery
关键词
胸椎
脊髓损伤
自噬
大鼠
Thoracic vertebrae
Spinal cord injuries
Autophagy
Rats
