摘要
目的探讨早期乳腺癌和乳腺良性疾病的乳管灌洗液细胞学涂片检查与RASSF1A基因甲基化检测敏感性及特异性的差异以及后者在早期乳腺癌中的诊断意义。方法回顾性分析以乳头溢液为表现的已接受手术治疗的乳腺良或恶性肿瘤患者40例,术前均行乳管灌洗液细胞学涂片检查,并运用甲基化特异性PCR方法对40例患者的乳管灌洗液标本进行RASSF1A基因甲基化的检测,分析对比两种检查方法的差异。结果乳管灌洗液的细胞学涂片检查对早期乳腺癌诊断的敏感性为24%,特异性为93.3%。RASSF1A甲基化诊断早期乳腺癌敏感性为80%,特异性为80%。相比细胞学检查RASSF1A甲基化检测对以病理性乳头溢液为表现的乳腺癌敏感性和特异性均较高,差异有统计学意义(P<0.05)。结论应用甲基化荧光定量PCR检测乳管灌洗液标本RASSF1A的甲基化可作为早期乳腺癌的术前诊断参考依据。
Objective To investigate the difference between cytological smear of breast duct lavage fluid and methylation of RASSF1A gene in early breast cancer and benign breast disease. Methods The sensitivity and specificity of preoperative cytological smear examination of breast duct lavage fluid in 40 patients with benign or malignant breast tumors with nipple discharge were retrospectively analyzed.Simultaneously,the methylation specific PCR method was used to analysis RASSF1A gene methylation in 40 cases of patients with breast duct lavage fluid.And then we analyzed the differences of two detection methods. Results The sensitivity and specificity of cytological smear of the fluid were 24% and 93.3%,respectively.Methylation of RASSF1A accounted for 80% of early breast cancer tissue,20% of benign breast disease.The diagnostic sensitivity and specificity were both 80%.Methylation of RASSF1A was more sensitive and specific to breast cancer with pathological nipple discharge than cytology.The difference between methylation of RASSF1A gene and cytological diagnosis of pathological nipple discharge was statistically significant( P <0.05). Conclusion The detection of methylation of RASSF1A gene by quantitative PCR could be used as a reference basis of preoperative diagnosis for early breast cancer.
作者
于海静
连臻强
YU Haijing;LIAN ZhenQiang(Breast Disease Center,Guangdong Women and Children’s Hospital,Guangzhou 511400,China)
出处
《济宁医学院学报》
2019年第5期305-308,314,共5页
Journal of Jining Medical University
基金
广东省医学科学技术研究基金项目(2017011).
