摘要
背景胃癌(gastric cancer,GC)是严重危害人体健康的恶性肿瘤,其发病率和死亡率分别占中国恶性肿瘤的第2位和第1位,早期诊断困难,因此,寻找GC诊断的新标志物对于提高GC的早期发现率及改善患者预后至关重要.目前,吞噬细胞运动蛋白1(engulfment and cellmotility 1,ELMO1)基因甲基化在GC中诊断价值研究鲜有报道.目的探讨ELMO1基因甲基化与GC的关系,旨在为GC早期诊断提供新思路.方法选取海南省肿瘤医院内镜中心2017-01/2018-08诊治的慢性非萎缩性胃炎20例、慢性萎缩性胃炎20例、GC37例(早期GC15例,进展期GC22例),胃镜检查同时收集胃液及活组织检查收集病理组织标本.通过甲基化特异聚合酶链反应检测三组患者ELMO1基因甲基化水平,并进行组间对比分析,并分析ELMO1基因甲基化与GC的发生、分期及转移的关系.结果ELMO1基因甲基化率在病理组织DNA中依次为慢性浅表性胃炎组0%,慢性萎缩性胃炎20%,GC组93.3%,差异显著(P<0.01);在胃液DNA中依次为:慢性浅表性胃炎组0%,慢性萎缩性胃炎组12.3%,GC组76.7%,差异显著(P<0.05).癌旁组织DNA中ELMO1基因甲基化率为96.7%,与GC组比较差异不显著(P>0.05);早期GC与进展期GC患者胃液中ELMO1基因甲基化率分别为73.3%、80.0%,两者组织中ELMO1基因甲基化率分别为86.7%、100%,两者在胃液及组织中比较均无显著差异(P>0.05).结论GC患者病理组织及胃液DNA中ELMO1基因启动子区均呈高甲基化状态,并有较高的一致性,并且在早期GC中即明显升高,ELMO1基因甲基化可作为GC早期诊断的分子靶标,并且胃液可用于ELMO1基因甲基化检测的良好临床标本.
BACKGROUND Gastric cancer(GC)is a malignant tumor that seriously endangers human health.Its morbidity and mortality rank second and first,respectively,among malignant tumors in China.It is difficult to diagnose early.Therefore,finding new markers for GC diagnosis is very important to improve the early detection rate and prognosis of GC.At present,there are few reports on the diagnostic value of engulfment and cell motility 1(ELMO1)gene methylation in GC.AIM To explore the relationship between the methylation of ELMO1 gene and GC in order to provide new ideas for early diagnosis of this malignancy.METHODS From January 2017 to August 2018,20 cases of chronic superficial gastritis,20 cases of chronic atrophic gastritis,and 37 cases of GC(15 cases of early GC and 22 cases of advanced GC)were selected.The gastric juice and biopsy were collected at the same time.Methylationspecific polymerase chain reaction was used to detect the methylation level of ELMO1 gene in the three groups,and the correlation between ELMO1 gene methylation and the occurrence,stage,and metastasis of GC was analyzed.RESULTS The methylation rate of ELMO1 gene in tissues and gastric juice of chronic superficial gastritis,chronic atrophic gastritis,and GC were 0%,20.5%,and 93.3%(P<0.01),and 0%,12.3%,and 76.7%(P<0.05),respectively.The methylation rate of ELMO1 gene in adjacent tissue DNA was 96.7%,which was not significantly different from that in the GC group(P>0.05).The methylation rates of ELMO1 gene in gastric juice of patients with early GC and advanced GC were 73.3%and 80.0%,respectively.The methylation rates of ELMO1 gene in the two tissues were 86.7%and 100%,respectively.There was no significant difference between them in both gastric juice and tissues(P>0.05).CONCLUSION The promoter region of ELMO1 gene in gastric juice DNA and pathological tissues of patients with GC is hypermethylated,which has high consistency.ELMO1 gene methylation can be used as a molecular target for early diagnosis of GC,and gastric juice can be used as a good clinica
作者
宋健
黎萍
袁桂红
贾真
张荣琳
王发宝
钟国柄
李依倪
钟敦璟
Jian Song;Ping Li;Gui-Hong Yuan;Zhen Jia;Rong-Lin Zhang;Fa-Bao Wang;Guo-Bing Zhong;Yi-Ni Li;Dun-Jing Zhong(Department of Digestive Endoscopy,Hainan Cancer Hospital,Haikou 570123,Hainan Province,China;Department of Anesthesiology,Hainan Cancer Hospital,Haikou 570123,Hainan Province,China;Department of Pathology,Hainan Cancer Hospital,Haikou 570123,Hainan Province,China;Central Laboratory of Hainan Cancer Hospital,Haikou 570123,Hainan Province,China)
出处
《世界华人消化杂志》
CAS
2019年第17期1055-1061,共7页
World Chinese Journal of Digestology
基金
海南省科技厅社会发展重点研发项目,No.ZDYF2017118~~
关键词
ELMO1基因
甲基化
胃癌
胃液
诊断
ELMO1
Methylation
Gastric carcinoma
Gastric juice
Diagnosis
