摘要
目的评价α7烟碱型乙酰胆碱受体(α7nAChR)在胫骨折断老龄大鼠术后认知功能障碍中的作用.方法清洁级健康雄性SD大鼠150只,18~22月龄,体重440~580 g,采用随机数字表法分为5组(n=30):对照组(C组)、假手术组(S组)、胫骨折断组(T组)、生理盐水组(N组)和α7nAChR激动剂PUN282987组(P组).C组不做任何处理;S组腹腔注射10%水合氯醛0.4 ml/100 g;T组采用胫骨折断术;P组于胫骨折断前5 min腹腔注射PUN2829872.4 mg/kg;N组胫骨折断前5 min给予腹腔注射等量生理盐水.于术后7 d进行Morris水迷宫实验;于术后1、3和7 d时采用HE染色观察海马CA3区病理学结果;采用Western blot法检测α7nAChR、胆碱乙酰转移酶(ChAT)、TNF-α和IL-1β的表达.结果与C组比较,T组、N组和P组术后逃避潜伏期和游泳路径延长,术后各时点海马α7nAChR、ChAT、TNF-α和IL-1β表达上调(P<0.05),S组上述指标差异无统计学意义(P>0.05);与T组比较,P组术后逃避潜伏期和游泳路径缩短,术后各时点海马α7nAChR和ChAT表达上调,TNF-α和IL-1β表达下调(P<0.05),N组上述指标差异无统计学意义(P>0.05),P组海马组织病理学损伤减轻.结论α7nAChR拮抗参与了胫骨折断老龄大鼠术后认知功能障碍的形成.
Objective To evaluate the role of α7 nicotinic acetylcholine receptor (α7nAChR) in postoperative cognitive dysfunction in aged rats with tibial fracture. Methods One hundred and fifty clean-grade healthy male Sprague-Dawley rats, aged 18-22 months, weighing 440-580 g, were divided into 5 groups ( n=30 each ) using a random number table method: control group ( group C ), sham operation group ( group S), tibial fracture group ( group T), normal saline group ( group N) and α7nAChR agonist PUN282987 group (group P). Group C received no treatment. Ten percent chloral hydrate 0. 4 ml/100 g was injected intraperitoneally in group S. Group T underwent tibial fracture. PUN2829872. 4 mg/kg was in-traperitoneally injected at 5 min before tibial fracture in group P . The equal volume of normal saline was giv-en at 5 min before tibial fracture in group N. Morris water maze test was performed at day 7 after surgery. At days 1, 3 and 7 after surgery, the pathological changes of the hippocampal CA3 region were observed by haematoxylin and eosin staining, and the expression of α7nAChR, choline acetyltransferase ( ChAT ), tumor necrosis factor-α( TNF-α) and interleukin-1β( IL-1β) in the hippocampal CA3 region was measured by Western blot. Results Compared with group C, the postoperative escape latency and swimming dis-tance were significantly prolonged, and the expression of α7nAChR, ChAT, TNF-α and IL-1β was up-regulated at each time point after operation in T, N and P groups ( P<0. 05), and no significant change was found in the parameters mentioned above in group S ( P>0. 05). Compared with group T, the postoper-ative escape latency and swimming distance were significantly shortened, and the expression of α7nAChR and ChAT was up-regulated and the expression of TNF-α and IL-1β was down-regulated at each time point after operation in group P ( P<0. 05), no significant change was found in the parameters mentioned above in group N ( P>0. 05), and the pathological changes of the hippocampal CA3 region were significantl
作者
林旭
李洪玲
刘晨
李肖璇
王彬
陈怀龙
孙立新
毕燕琳
时飞
王明山
Lin Xu;Li Hongling;Liu Chen;Li Xiaoxuan;Wang Bin;Chen Huailong;Sun Lixin;Bi Yanlin;Shi Fei;Wang Mingshan(Department of Anesthesiology,Municipal Hospital of Qingdao,Qingdao 266000,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2019年第6期699-702,共4页
Chinese Journal of Anesthesiology
关键词
胆碱能激动剂
老年人
认知障碍
手术后并发症
Cholinergic agonists
Aged
Cognition disorders
Postoperative complications
