摘要
毕赤酵母作为一种重要的表达外源蛋白的宿主,提高其外源蛋白的分泌量非常有必要。近年来很多学者报道了与毕赤酵母外源蛋白分泌相关的基因、蛋白质,同时毕赤酵母基因组的公布加快了这方面的研究进展。文章根据外源蛋白分泌的途径,分步骤地总结了涉及的基因和蛋白,有利于分析控制蛋白分泌效率的具体步骤,为构建更加高效的毕赤酵母表达系统提供参考。
Komagataella phaffii, as an important host for heterologous protein expression is very necessary to improve for its secretory capacity of heterologous protein. Recently, many scholars have reported heterologous protein secretory related genes and proteins from K. phaffii. And the announcement of K. phaffii genome sequence has promoted the progress of the studies in this aspect. This article summarizes the genes and proteins related to protein secretory pathway according to the steps of protein secretory, and benefits to analyze the concrete steps for controlling the secretion effects of the protein, in order to establish a more effective expression system of K. phaffii provide a foundation.
作者
刘冰
黄志强
郑潇
张建国
LIU Bing;HUANG Zhi-qiang;ZHENG Xiao;ZHANG Jian-guo(Inst. of Food Sci. & Engin.,Schl. of Med. Inst. & Food Engin.,Uni. of Shanghai for Sci. & Technol.,Shanghai 200093)
出处
《微生物学杂志》
CAS
CSCD
2019年第4期83-90,共8页
Journal of Microbiology
基金
国家自然科学基金面上项目(31870045,41601315)
关键词
毕赤酵母
外源蛋白
分泌途径
分子伴侣
蛋白转运
Komagataella phaffii
heterologous protein
secretory pathway
molecular companion
protein transport
