摘要
目的:观察雌激素及麦粒灸对大鼠薄型子宫内膜的修复作用,初步探讨其可能的机制。方法:将40只健康SPF级性成熟雌性SD大鼠按随机数字表法分为正常组、模型组、雌激素组、麦粒灸组,每组10只。除正常组外,其余3组大鼠均于动情期建立薄型子宫内膜模型。正常组不予干预,模型组于造模后次日予2 m L0.9%Na Cl溶液灌胃,雌激素组于造模后次日予2 m L雌二醇灌胃,麦粒灸组于造模后次日麦粒灸"关元""肾俞",每穴7壮。均每日1次,于3个动情周期后的动情期取材,HE染色观察子宫内膜厚度及其形态;免疫组化法检测子宫内膜组织中波形蛋白、角蛋白及血管内皮生长因子(VEGF)的表达;Western blot法检测子宫内膜组织中同源框转录因子HOXA10、白血病抑制因子(LIF)蛋白表达。结果:模型组子宫内膜厚度较正常组显著变薄(P<0.01);与模型组比较,雌激素组、麦粒灸组子宫内膜厚度明显增加(P<0.05,P<0.01);麦粒灸组子宫内膜厚度高于雌激素组,差异无统计学意义(P>0.05)。模型组大鼠子宫内膜角蛋白、波形蛋白、VEGF表达量与正常组比较显著降低(P<0.01);与模型组比较,雌激素组及麦粒灸组大鼠子宫内膜角蛋白、波形蛋白、VEGF表达量显著增加(P<0.01);麦粒灸组子宫内膜中角蛋白、波形蛋白、VEGF的表达量均高于雌激素组,但差异无统计学意义(P>0.05)。模型组大鼠子宫内膜HOXA10、LIF蛋白表达水平较正常组显著降低(P<0.01),与模型组比较,雌激素组及麦粒灸组大鼠子宫内膜HOXA10、LIF蛋白表达水平明显增加(P<0.05)。结论:麦粒灸可以通过上调大鼠子宫内膜中角蛋白、波形蛋白及VEGF的表达从而改善子宫内膜的厚度,并且可提高HOXA10、LIF等子宫内膜容受性相关因子的水平,改善子宫内膜容受性,发挥修复作用。
Objective To observe the repairing effects of estrogen and wheat-grain moxibustion on thin-type endometrium in rats and to explore its possible mechanism. Methods Forty healthy SPF-grade adult female SD rats were randomly divided into a normal group, a model group, an estrogen group and a moxibustion group according to random number table method, 10 rats in each group. The model of thin-type endometrium was established during estrous period in all the groups except for the normal group. No intervention was given in the normal group. The intragastric administration of 2 m L of 0.9% sodium chloride solution was applied the next day after modeling in the model group. The intragastric administration of 2 m L of estradiol was given the next day after modeling in the estrogen group. The wheat-grain moxibustion was given at "Guanyuan"(CV 4) and "Shenshu"(BL 23) the next day after modeling in the moxibustion group, 7 moxa cones for each acupoint. The treatment in 3 groups was given once a day. After three estrous cycles, the samples were collected during estrous period;the thickness and morphology of endometrium were observed by HE staining;the expressions of vimentin, keratin and vascular endothelial growth factor(VEGF) in endometrium tissue were detected by immunohistochemistry;the expressions of HOXA10 and LIF in endometrium tissue were detected by Western blot. Results The endometrial thickness in the model group was significantly thinner than that in the normal group(P<0.01);compared with the model group, the endometrial thickness in the estrogen group and the moxibustion group were increased significantly(P<0.05, P<0.01);the endometrial thickness in the moxibustion group was insignificantly higher than that in the estrogen group(P>0.05). The expressions of keratin, vimentin and VEGF in endometrium in the model group were significantly lower than those in the normal group(P<0.01);compared with the model group, the expressions of keratin, vimentin and VEGF in endometrium in the estrogen group and the moxibustion grou
作者
胡玉姣
夏良君
姚兵
陈莉
程洁
夏有兵
HU Yu-jiao;XIA Liang-jun;YAO Bing;CHEN Li;CHENG Ji;XIA You-bing(Nanjing University of CM. Nanjing 210029, Jiangsu Province, China;Reproductive Medicine Center, Nanjing General Hospital of Nanjing Military Region)
出处
《中国针灸》
CAS
CSCD
北大核心
2019年第9期963-969,共7页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金面上项目：81473767
关键词
薄型子宫内膜
雌二醇
麦粒灸
子宫内膜容受性
thin-type endometrium
estradiol
wheat-grain moxibustion
endometrial receptivity
