摘要
目的:探讨高良姜、大高良姜、红豆蔻、草豆蔻、益智等5 味山姜属中药不同极性部位对胃溃疡寒证大鼠交感神经-肾上腺系统机能的影响。方法:取SD大鼠,均灌胃4 ℃知母水煎液、冰醋酸溶液复制胃溃疡寒证模型,并于造模后随机分为模型组、附子理中丸组(阳性对照,9.0 g/kg)、西咪替丁组(阳性对照,0.003 3 g/kg)以及5 种中药石油醚、乙酸乙酯、正丁醇、水萃取部位低、高剂量组(以下简称“高石低/高”“大石低/高”“红石低/高”“草石低/高”“益石低/高”“高乙低/高”“大乙低/高”“红乙低/高”“草乙低/高”“益乙低/高”“高正低/高”“大正低/高”“红正低/高”“草正低/高”“益正低/高”“高水低/高”“大水低/高”“红水低/高”“草水低/高”“益水低/高”,剂量分别为0.064/0.256、0.032/0.128、0.008/0.032、0.075/0.3、0.1/0.4、0.064/0.256、0.108/0.432、0.16/0.64、0.064/0.25、0.125/0.5、0.056/0.224、0.108/0.432、0.08/0.32、0.2/0.8、0.3/1.2、0.14/0.56、0.032/0.128、0.028/0.112、0.05/0.2、0.087/0.348 g/kg,以萃取部位质量计),每组10只;另设空白组(常温水)。造模次日,空白组和模型组大鼠均灌胃等容常温水,各给药组大鼠灌胃相应药液2 mL/100 g,q12 h,共给药4 次。末次给药后,采用酶联免疫吸附测定法检测各组大鼠尿液中17-羟皮质类固醇(17-OHCS)和儿茶酚胺类(CAs)物质[肾上腺素(A)、去甲肾上腺素(NE)、多巴胺(DA)]以及血清中促肾上腺皮质素(ACTH)、多巴胺-β-羟化酶(D-β-H)的含量。结果:与空白组比较,模型组大鼠尿液中17-OHCS、A、NE、DA以及血清中ACTH、D-β-H 含量均显著降低(P<0.01)。与模型组比较,不同给药组大鼠尿液17-OHCS(附子理中丸组、西咪替丁组以及高石低/高、大石高、红石高、草石高、益石高组,5 种药材乙酸乙酯部位各剂量组,大正高、红正高、草正高、益正高组,高水高、大水高、红
OBJECTIVE:To investigate the effects of different polar parts of 5 kinds of Alpinia on the function of sympathetic-adrenal system in gastric ulcer model rats with cold syndrome,such as the root of Alpinia officinarum,the rhizome and fruit of Alpinia galangal,the seed of Alpinia katsumadai,and the fruit of Alpinia oxyphylla. METHODS:SD rats were given Anemarrhena asphodeloides decoction at 4 ℃ and Glacial acetic acid solution intragastrically to induce gastric ulcer model with cold syndrome;the model rats were randomly divided into model group,Fuzi lizhong pills group(positive control,9.0 g/kg), cimetidine group(positive control,0.003 3 g/kg),low-dose and high-dose groups of petroleum ether,ethyl acetate,n-butanol and water extraction parts from 5 medicinal materials (hereinafter referred to as “gaoshidi”“gaoshigao”“dashidi”“dashigao”“hongshidi”“hongshigao”“caoshidi”“caoshigao”“yishidi”“yishigao”“gaoyidi”“gaoyigao”“dayidi”“dayigao”“hongyidi”“hongyigao”“caoyidi”“caoyigao”“yiyidi”“yiyigao”“gaozhengdi”“gaozhenggao”“dazhengdi”“dazhenggao”“hongzhengdi”“hongzhenggao”“caozhengdi”“caozhenggao”“yizhengdi”“yizhenggao”“gaoshuidi”“gaoshuigao”“dashuidi”“dashuigao”“hongshuidi”“hongshuigao”“caoshuidi”“caoshuigao”“yishuidi”“yishuigao”,0.064/0.256,0.032/ 0.128,0.008/0.032,0.075/0.3, 0.1/0.4,0.064/0.256,0.108/0.432,0.16/0.64,0.064/0.25,0.125/0.5,0.056/0.224,0.108/0.432,0.08/0.32,0.2/0.8,0.3/1.2,0.14/0.56, 0.032/0.128,0.028/0.112,0.05/0.2,0.087/0.348 g/kg,by mass of extraction parts),with 10 rats in each group;and the blank group(normal temperature water)was set up. Next day after modeling,blank group and model group were given constant volume of normal temperature water intragastrically;administration group was given relevant solution 2 mL/100 g intragastrically,q12 h, 4 times in total. After last medication,urine contents of 17-OHCS,CAs substances(A,NE,DA)and serum conten
作者
柳俊辉
丘海冰
谢鹏
苏善美
李明芳
陈俊其
尹优
秦华珍
LIU Junhui;QIU Haibing;XIE Peng;SU Shanmei;LI Mingfang;CHEN Junqi;YIN You;QIN Huazhen(Collegeof Pharmacy,Guangxi University of TCM,Nanning 530200,China)
出处
《中国药房》
CAS
北大核心
2019年第18期2518-2524,共7页
China Pharmacy
基金
国家自然科学基金资助项目(No.81360646)
广西壮族自治区科学技术厅广西重点实验室建设项目(No.17-259-20)
