摘要
核桃破碎后经体积分数为70%乙醇提取,乙酸乙酯萃取、浓缩后,经大孔树脂、MCI、ODS等不同层析柱分离。采用MTT法和试剂盒法,以乳鼠心肌细胞缺血/再灌注损伤模型检测各分离组分抗心肌缺血活性。结果从核桃中分离得到5,10-二羟色胺和Shepherdine化合物,其中5,10-二羟色胺能显著提高心肌细胞存活率,减少受损细胞心肌酶(LDH、CK)的释放,对心肌细胞具有保护作用。
The functional components of anti-myocardial ischemic activity in walnut were studied.The walnuts were squashed and then extracted by 70%ethanol,and ethyl acetate.The ethyl acetate phase was concentrated and then separated by macroporous resin,MCI,and ODS columns.MTT assay and kit method were applied,along with the model of myocardial ischemia/reperfusion injury to detect the anti-ischemic activity of each isolated component.The results showed that 5,10-dihydroxytryptamine and Shepherdine were separated from walnuts for the first time.Moreover,5,10-dihydroxytryptamine could significantly maintain the viability of myocardial cell and reduce the enzymes(LDH/CK)released by ischemia/reperfusion injury,having significant protective effects on myocardial ischemia/reperfusion injury.
作者
陈晨
徐德平
金蒙
张晓娟
CHEN Chen;XU De-ping;JIN Meng;ZHANG Xiao-juan(Food Faculty of Jiangnan University, Wuxi, Jiangsu 214122, China)
出处
《食品与机械》
北大核心
2019年第8期167-171,共5页
Food and Machinery
基金
江苏高校品牌专业建设工程资助项目(编号:PPZY2015A052)
云南省重大科技专项计划项目(编号:2018ZG003)
