摘要
为建立羊乳中牛乳成分的检测方法,采用西北农林科技大学动物医学院免疫学实验室制备并保存的牛α酪蛋白单克隆抗体,分别建立间接ELISA和间接阻断ELISA检测方法,并对两种方法的灵敏度及重复性进行试验。结果显示,建立的间接ELISA方法测定羊乳中牛α酪蛋白的检测灵敏度为1.3μg/mL,变异系数为2.7%;建立的间接阻断ELISA方法测定羊乳中牛α酪蛋白标准曲线的灵敏度为13.7 ng/mL,变异系数为2.9%。表明间接阻断ELISA标准曲线具有更高的检测灵敏度,对羊乳中牛乳成分的检测更准确。
In order to detect bovine milk components in goat milk,mAb of anti-bovine α-casein prepared by Immunology Laboratory of Northwest A&F University was used to establish an indirect ELISA and an indirect-blocking ELISA method.The results showed that,the sensitivity of indirect ELISA for determining bovine α casein content in goat milk is 1.3μg/mL,and the coefficient of variation(CV)is 2.7%.The sensitivity of indirect-blocking ELISA for determining bovine α casein content in goat milk is 13.7 ng/mL,and the CV is 2.9%.The indirect-blocking ELISA has higher detection sensitivity,and it is more accurate to determine bovine milk components in goat milk.
作者
王毅
刘阿华
李言言
高洋
宁梦夏
马文涛
陈德坤
WANG Yi;LIU A-hua;LI Yan-yan;GAO Yang;NING Meng-xia;MA Wen-tao;CHEN De-kun(College of Veterinary Medicine, Northwest A& F University,Yangling, Shaanxi,712100, China)
出处
《动物医学进展》
北大核心
2019年第9期19-23,共5页
Progress In Veterinary Medicine
基金
陕西省重点产业创新链(群)项目(2018ZDCXL-NY-01-06)
咸阳市重大科技专项计划项目(2017K01-34)
青海省重点研发与转化计划项目(NMSY-2018-07)
