摘要
建立可检测水牛乳和羊乳中掺伪牛乳的双抗夹心酶联免疫检测方法。分别以牛Ig G和牛Ig G的Fc片段为抗原,制备抗牛Ig G的多克隆和高特异性单克隆抗体,并将以此蛋白作为包被抗体,构建双抗夹心ELISA方法。该方法标准曲线的IC50为4.3μg/m L,方法的灵敏度为0.1μg/m L。添加回收率在95%~105%之间,相对标准偏差小于12%。该方法无需复杂的前处理,检测时间为60 min,能准确检测水牛乳和羊乳中掺伪牛乳,避免了传统免疫方法在乳制品掺伪检测上的假阳性。
The aim of the research was to develop sandwich-antibody enzyme linked immtmosorbent assay （ELISA） for adulteration of bovine milk into sheep, goat and buffalo milk. Bovine IgG and its Fc fragment of bovine IgG were used as antigen respectively to prepare polyclonal and monoclonal antibody against bovine IgG. The sandwich-antibody ELISA was established which used monoclonal antibody for the coating antibody and polyclonal antibody enzyme labeled for second antibody. The IC50 of standard curve was 4.3 ng/mL. The sensitivity of the method were 0.1 ng/mL. The recoveries fortified which were between 95%~105%. The RSD were less than 12%. The method need only 60 min and could recognize the bovine milk in buffalo and goat milk without complex prelreatment of samples, which could avoid rnisjudgement in analysis for adulteration.
出处
《食品工业》
北大核心
2015年第5期191-194,共4页
The Food Industry
关键词
羊乳
水牛乳
掺伪
酶联免疫分析(ELISA)
buffalo milk
goat milk
adulteration
enzyme-linked immuno sorbent assay（ELISA）
