摘要
【目的】评价河西走廊‘贵人香’7个葡萄砧穗的抗寒性强弱.【方法】酿酒葡萄品种‘贵人香’与‘5BB’‘5C’‘420’‘3309C’‘101-14MG’‘110R’‘1103P’7个砧木进行嫁接,采集一年生休眠枝条,分别在-15、-20、-25、-30、-35℃下进行人工低温胁迫处理,以4℃贮藏的枝条为对照,测定了各砧穗组合的相对电导率、丙二醛、游离脯氨酸、可溶性糖和可溶性蛋白含量等抗寒相关指标的变化,结合主成分分析和聚类分析法对砧穗组合的抗寒性进行综合评价.【结果】随处理温度的降低,各葡萄砧穗组合的相对电导率、丙二醛、游离脯氨酸和可溶性蛋白含量总体呈现出‘降低-升高-降低’的变化趋势,而可溶性糖含量总体呈现出‘升高-降低-升高’的变化趋势.其中砧穗组合IR/5C的相对电导率在-30^-35℃温度范围下降幅度大于其它组合,而在温度为-20^-25℃时,游离脯氨酸和可溶性糖含量显著高于其他组合;IR/1103P在-10^-15℃和-25^-35℃温度范围内可溶性糖和可溶性蛋白含量显著下降.【结论】综合各项生理指标,结合主成分分析法、聚类分析法以及相关性分析,科学的鉴定了河西走廊葡萄产区葡萄砧穗组合的抗寒性.结果显示抗寒性由强到弱为:IR/1103P>IR/5BB>IR/420>IR/110R>IR/5C>IR/101-14MG>IR/3309C.
【Objective】 To evaluate the cold resistance of 7 grape rootstock-scion combinations of Italian Riesling in Hexi Corridor.【Method】‘Italian Riesling’ grape was grafted onto 7 different rootstocks,including ‘5 BB’‘5 C’‘420’‘3309 C’‘101-14 MG’‘110 R’‘1103 P’.The one-year-old branches were collected and stored at -15 ℃,-20 ℃,-25 ℃,-30 ℃ and -35 ℃,with stored at 4℃ as the control. The cold resistance related indicators were determined as relative conductivity,the content of malondialdehyde(MDA),free proline,soluble sugar and soluble protein. The cold resistance of each grape rootstock-scion combinations was comprehensively evaluated by Subordinate function method.【Result】 With the decrease of temperature,the relative conductivity,MDA,free proline and soluble protein contents of the branches of each grape rootstock-scion combinations showed a tendency of ‘decreasing,increasing and decreasing’,while the total soluble sugar content showed a trend of ‘increasing,decreasing and increasing’. The relative conductivity of IR/5 C was lower than that of other rootstock-scion combinations in the temperature range of -30~35 ℃,while the content of proline and soluble sugar was higher than that of others at the temperature of -20~25 ℃. The soluble sugar and soluble protein contents of rootstock-scion combination IR/1103 P decreased significantly in the temperature range of -10^-15 ℃ and -25^-35 ℃.【Conclusion】 The results of physiological indicators,combining principal component analysis,cluster analysis and correlation analysis suggested that the cold resistance ranged from strong to weak: IR/5 C> IR/420> IR/110 R> IR/3309 C> IR/101-14 MG> IR/5 BB>IR/1103 P.
作者
任静
郝燕
白耀栋
朱燕芳
张思成
REN Jing;HAO Yan;BAI Yao-dong;ZHU Yan-fang;ZHANG Si-cheng(Institute of Fruit and Floriculture Research,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;Hexi Comprehensive Development Department,Bailong River Forestry Administration Bureau,Gaotai734300,China;Cash Crop Technical Advice Station of Honggu,Lanzhou 730080,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2019年第4期60-68,共9页
Journal of Gansu Agricultural University
基金
国家葡萄产业技术体系兰州综合试验站项目(CARS-29-23)
甘肃省酿酒葡萄专项资金项目“甘肃河西走廊酿酒葡萄产区树形优化及机械化栽培配套技术研究与示范”(甘发改投资[2015]1096号)
甘肃河西走廊酿酒葡萄抗寒栽培技术研究与示范项目(甘商务财务发[2018]357号)
关键词
'贵人香’葡萄
砧穗组合
主成分分析法
聚类分析
抗寒性
河西走廊
Hexi Corridor
‘Italian Riesling’ grape
rootstock-scion combination
principal component analysis
cluster analysis
cold resistance
