摘要
【目的】分析48份杨桃种质的遗传多样性,为杨桃种质资源的鉴定保护及开发利用提供理论依据。【方法】从60条SCoT引物中筛选出扩增条带清晰稳定、多态性丰富的引物,对从国内外收集的48份杨桃种质材料进行PCR扩增,统计分析电泳图谱,利用Popgene 1.32计算其遗传多样性参数,采用NTSYSpc 2.1计算种质间的遗传相似系数和遗传距离,利用UPGMA法进行聚类分析,并根据遗传相似系数进行主成分分析。【结果】从60条SCoT引物中筛选出的11条引物共扩增出115条条带,其中多态性条带102条,占总条带数的88.7%,每条SCoT引物扩增的总条带数(TNB)和多态性条带数(NPB)分别为10.45和9.27条,多态性比率(PPB)为70.00%~100.00%,平均为88.84%;多态性信息量(PIC)、有效等位基因数(Ne)、Nei’s基因多样性指数(H)和Shannon’s信息指数(I)的平均值分别为0.77、1.7758、0.4229和0.6061。48份杨桃种质间的遗传相似系数为0.4957~0.9217,平均为0.6841。聚类分析结果显示,在遗传相似系数0.6618处可将48份杨桃种质划分为三大类群,第Ⅰ类群均为甜杨桃种质,第Ⅱ类群以酸杨桃种质为主,第Ⅲ类群以甜杨桃种质为主。主成分分析结果与聚类分析结果基本一致,均与杨桃的果实风味和种质来源高度相关。【结论】杨桃种质资源具有较丰富的遗传多样性,且筛选获得的SCoT引物对杨桃种质资源有较高的多态性检测率,适用于杨桃种质资源鉴别及亲缘关系分析。
【Objective】In order to provide theoretical support and reference for the identification,protection,development and utilization of Averrhoa carambola L. germplasm resources,the present study analyzed the genetic diversity of 48 A. carambola germplasms.【Method】SCoT primers with rich polymorphism and clear amplified bands were screened out from 60 primers,the 48 A. carambola germplasm resources collected from home and abroad were amplified by PCR. The electrophoretogram was analyzed,and genetic diversity parameters were calculated by Popgene 1.32. Then the genetic similarity coefficient and genetic distance between different germplasms were calculated using NTSYSpc 2.1. The unweighted pair-group method with arithmetic mean(UPGMA)method was used for cluster analysis. Based on the genetic similarity,principal coordinate analysis was also carried out.【Result】A total of 115 bands were amplified from 11 primers which screened out from 60 SCoT primers,including 102 polymorphic bands,accounting for 88.7% of the total. The total number of bands(TNB)and polymorphic bands(NPB)amplified from each primer were 10.45 and 9.27,respectively. The percentage of polymorphism bands(PPB)was 70.00%-100.00%,with an average of 88.84%. The mean value of polymorphism information content(PIC),effective number of alleles(Ne),Nei’s gene diversity(H)and Shannon’s information index(I)were 0.77,1.7758,0.4229 and 0.6061,respectively. The genetic similarity coefficient between different A. carambola germplasms was 0.4957-0.9217,and the average was 0.6841. According to the results of cluster analysis,48 A.carambola germplasms could be divided into three groups at the genetic similarity of 0.6618. All the A. carambola germplasms in groupⅠand Ⅲ belonged to sweet A. carambola,while groupⅡwas mainly acid A. carambola. The results of principal component analysis and cluster analysis were basically consistent,and both were highly related to the fruit flavor and germplasm sources.【Conclusion】The genetic diversity of A. carambola ge
作者
欧景莉
朱杨帆
陈豪军
周俊岸
陈燕
何江
宁琳
潘祖建
甘卫堂
OU Jing-li;ZHU Yang-fan;CHEN Hao-jun;ZHOU Jun-an;CHEN Yan;HE Jiang;NING Lin;PAN Zu-jian;GANWei-tang(Guangxi Subtropical Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530001,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2019年第8期1680-1687,共8页
Journal of Southern Agriculture
基金
农业农村部物种品种资源保护(热带作物)项目(151821301354052707)
广西科技重大专项(桂科AA17204045,桂科AA17204045-4)
广西自然科学基金项目(2016GXNSFBA380111)
关键词
杨桃
遗传多样性
SCoT标记
聚类分析
主成分分析
Averrhoa carambola L.
genetic diversity
SCoT marker
clustering analysis
principal component analysis
