摘要
以三苯甲烷类染料孔雀石绿为唯一碳源,通过连续传代驯化,拟获得能够降解孔雀石绿的富集培养物。运用高通量测序对富集培养物进行了微生物群落区系变化分析,获得了与降解相关的微生物信息,进一步分离纯化到了 1 株孔雀石绿高效降解菌株。该菌 7 d 内对 50 mg/L 孔雀石绿降解效率达 91.2%。经初步鉴定,该菌为 Pseudomonas 菌属菌株,并命名为 Pseudomonasveronii JW3-6。对该菌进行了降解条件优化,结果表明菌株最适降解温度为 30℃,pH 为 7,接种浓度为 1×10^7 CFU/mL。
Using triphenylmethane dye malachite green as the sole carbon source,the enriched culture degrading malachite green was obtained by continuous passage domestication. High-throughput sequencing was applied to analyze the microbial community change of the enriched culture,and the main microbial information related to degradation was acquired,and further an efficient strain degrading malachite green was isolated and purified. This strain degraded 91.2% of 50 mg/L malachite green within 7 days. The strain was initially identified as a Pseudomonas,and named as Pseudomonas veronii JW3-6.The optimized condition of degradation by this strain were temperature 30℃,pH 7, and inoculation concentration 1×10^7 CFU/mL.
作者
王亚妮
宋金龙
韩刚
穆迎春
江旭
王金耀
阮志勇
李乐
WANG Ya-ni;SONG Jin-long;HAN Gang;MU Ying-chun;JIANG Xu;WANG Jin-yao;RUAN Zhi-yong;LI Le(Shanghai Ocean University,Shanghai 201306;Chinese Academy of Fishery Sciences,Beijing 100141;Institute of AgriculturalResources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;Bayannaoer No. 1 Middle School,Bayannaoer 015002)
出处
《生物技术通报》
CAS
CSCD
北大核心
2019年第9期150-155,共6页
Biotechnology Bulletin
基金
中央级公益性科研院所基本科研业务费(2017C004)
国家自然科学基金项目(31700006)
关键词
孔雀石绿
高通量测序
微生物降解
假单胞菌
malachite green
high-throughput sequencing
microbial degradation
Pseudomonas
