摘要
筛选并鉴定出1株雌激素降解菌Acinetobacter sp.命名为DS1,对DS1降解17β-雌二醇实验条件进行优化,使用响应面法(RSM)对降解过程中底物浓度、接种量、培养温度和p H值进行了分析,构建响应面模型,并对反应条件进行优化。优化最佳降解条件为:17β-雌二醇量5.10 mg/L;接种量7.5%;p H值8.7;温度27℃。并进行试验验证,结果接近,响应面模型可有效描述降解过程,为降解工程应用和设计提供技术参考。
A strain of estrogen-degrading bacteria Acinetobacter sp.,named DS1,was screened from soil samples collected in proximity to a pharmaceutical factory in Beijing,and then was identified.To optimize the degradation of 17β-estradiol by DS1,a laboratory experiment was conducted by use of the response surface methodology(RSM)involving the parameters:substrate concentration,inoculation amount,culture temperature and pH value;those parameters were analyzed to construct a response surface model and the reaction conditions were optimized.Thereby,the optimum degradation condition was determined as follows:17β-estradiol was 5.10 mg/L,inoculation 7.5%,pH 8.7 and temperature 27℃;in addition,the optimized result was verified,which was indicating that RSM could well describe the degradation process,providing technical references for application and design of degradation engineering.
作者
靳申宝
马喆
田克俭
霍洪亮
JIN Shenbao;MA Zhe;TIAN Kejian;HUO Hongliang(Laboratory of Jilin Province for Water Pollution Control and Recycling Engineering,Northeast Normal University,Changchun 130117,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2019年第1期94-100,共7页
Environmental Science & Technology
基金
国家自然科学基金面上项目(51478096)
