摘要
目的探讨人体胸腰椎段椎骨显微骨硬度的分布特征及临床应用前景。方法选取3具新鲜成人尸体标本,取出其T11~L2椎骨后剔除软组织。将每一个椎骨分为椎体区和附件区,经高精慢速锯切割成若干厚约3 mm的骨组织切片,12块椎骨标本共计产生72个骨组织切片。应用维氏显微硬度测量仪测量骨组织切片不同区域皮质骨和松质骨的显微硬度值,每一个区域选取5个有效显微硬度值,全体有效值的平均值作为该区域的硬度值。结果12块椎骨共进行660次有效压痕实验并取得显微硬度值。(1)胸腰段椎休总体硬度为11.6~48.3 HV,其中皮质骨硬度为13.8~48.3(31.62±5.66)HV,松质骨硬度11.6~44.9(29.62±5.38)HV;(2)胸腰段椎体区和附件区皮质骨硬度分别为(29.99±5.27)HV和(32.92±5.63)HV,松质骨硬度分别为(28.44±4.79)HV和(30.81±5.71)HV,附件区均高于椎体区,差异均有统计学意义(t=5.098、2.011,P值均<0.05);(3)椎体区皮质骨硬度由高到低依次为下终板(33.94±4.31)HV、上终板(29.76±4.35)HV、外周终板(28.13±5.07)HV,下终板皮质的骨硬度高于上终板、外周终板,差异均有统计学意义(P值均<0.05);(4)附件区皮质骨中,硬度由高到低依次为椎弓根皮质(34.78±5.30)HV、上关节突(33.73±5.68)HV、椎板(33.15±5.28)HV、横突(31.69±5.37)HV和下关节突(31.26±5.91)HV,其中椎弓根皮质骨硬度与横突和下关节突相比,差异均有统计学意义(P值均<0.05)。结论本实验首次应用维氏显微硬度方法对人体胸腰椎段椎骨显微硬度的分布规律进行研究,发现胸腰椎段椎骨附件区皮质骨、松质骨骨硬度分别高于椎体区皮质骨、松质骨骨硬度,胸腰椎段T11~L2之间、不同标本的胸腰椎段的骨显微硬度存在差异,但分布规律相对一致,是微观结构、力学承载共同作用的结果,符合人体正常生理及负重功能。本研究结果可为3D打印制备具有梯度硬度的人工椎骨提供数据支持。
Objective To explore the distribution characteristics and clinical application prospects of micro-hardness of the human thoracolumbar segments.Methods Three fresh adult cadaver T11-L2 vertebrae specimens were selected and the soft tissue was removed.Each vertebra was divided into a vertebral body region and an attachment region.All of the bones were prepared in to a plurality of 3 mm bone tissue slices by using a high-precision slow saw and then sanded by sandpaper.A total of 72 bone tissue sections were generated from 12 vertebral specimens.The Vickers method was used to measure the microhardness values of cortical bone and cancellous bone in different areas of bone sections.Five effective microhardness values were selected for each region,and the average value of all effective values was used as the hardness value of the region.Results A total of 660 effective indentation experiments were performed on 12 vertebrae.(1)The total hardness of the thoracolumbar segment was 11.6-48.3 HV,including the average hardness of the cortical bone was 13.8-48.3(31.62±5.66)HV,and the average hardness of the cancellous bone was 11.6-44.9(29.62±5.38)HV.(2)The average hardness of the cortical bone in the vertebral body region and the accessory region were(29.99±5.27)HV and(32.92±5.63)HV,respectively.The average hardness of cancellous bone were(28.44±4.79)HV and(30.81±5.71)HV,respectively.The attachment area was higher than the vertebral body area,and the difference was statistically significant(t=5.098,2.011,all P values<0.05).(3)The hardness of the cortical bone in the vertebral body region from high to low was the lower endplate(33.94±4.31)HV,the upper endplate(29.76±4.35)HV,and the peripheral endplate(28.13±5.07)HV.The bone hardness of the lower endplate was higher than that of the upper endplate and the peripheral endplate.The difference was statistically significant(all P values<0.05).(4)In the cortical bone of the attachment area,the hardness from high to low was the pedicle cortex(34.78±5.30)HV,the upper articular
作者
李升
张晓娟
吴卫卫
刘国彬
王建朝
殷兵
胡祖圣
付蕾
张英泽
Li Sheng;Zhang Xiaojuan;Wu Weiwei;Liu Guobin;Wang Jianzhao;Yin Bing;Hu Zusheng;Fu Lei;Zhang Yingze(Department of Orthopaedic Surgery,the Third Hospital of Hebei Medical University,Key Laboratory of Biomechanics of Hebei Province,Shijiazhuang 050051,China)
出处
《中华解剖与临床杂志》
2019年第4期313-317,共5页
Chinese Journal of Anatomy and Clinics
基金
国家自然科学基金(81572125、81501934).
关键词
脊柱
胸腰椎
椎骨
骨硬度实验
维氏硬度
显微硬度
人体骨骼
Spine
Thoracolumbar spine
Vertebrae
Bone hardness test
Vickers hardness
Microhardness
Human skeleton
