摘要
目的对比无偿献血者HIV初筛检测策略及确证结果。方法选取溧阳分站2012年1月—12月32811例标本作为该次研究对象,所有无偿献血标本均采用酶联免疫吸附法(ELISA)法进行HIV抗体检测,如果检测结果有反应性,则按照《全国艾滋病检测技术规范》的要求,用2种试剂进行复试,若检测结果为1种或2种检测试剂均为有反应性,则将标本送常州CDC进行确诊。结果2012年1—12月送检标本8851例,抗-HIV阳性数7例、送检CDC标本数3例、确诊2例,确诊率为66.67%;2013年1—12月送检标本8293例,抗-HIV阳性数8例、送检CDC标本数6例、确诊3例,确诊率为50.00%;2014年1—12月送检标本8229例,抗-HIV阳性数5例、送检CDC标本1例,确诊率为20.00%;2015年1—12月送检标本7438例,抗-HIV阳性数4例、送检CDC标本4例、确诊1例,确诊率为25.00%。结论ELISA法可进行无偿献血HIV的初检,对于HIV抗体不确定的标本可结合核酸和抗体确诊。血站工作人员应做好献血者前健康咨询,尽可能将HIV感染者隔离在献血前,充分保证血液安全。
Objective To compare the HIV screening test strategy and confirm the results of unpaid blood donors. Methods A total of 32 811 specimens from January 2012 to December 2015 in Liyang sub-station were selected as the study subjects. All unpaid blood donation specimens were tested for HIV antibody by enzyme-linked immunosorbent assay (ELISA). If it is reactive, it will be retested with two kinds of reagents according to the requirements of the National AIDS Test Technical Specification. If the test results of one or two test reagents are reactive, the specimen will be sent to Changzhou CDC for diagnosis. Results From January to December 2012, the number of specimens was 8 851, 7 were anti-HIV positive, 3 were CDC specimens, and 2 were confirmed. The diagnosis rate was 66.67%;8 293 cases from January to December 2013 were examined, 8 cases were anti-HIV positive, 6 cases were CDC specimens, 3 cases were diagnosed, and the diagnosis rate was 50.00%;8 229 cases were sent from January to December 2014, anti-HIV positive number 5 cases, 1 case of CDC specimens were sent for examination, the diagnosis rate was 20.00%;7 438 cases were sent from January to December 2015, 4 cases were anti-HIV positive, 4 cases were CDC specimens, 1 case was confirmed, and the diagnosis was confirmed, and the rate was 25.00%. Conclusion The ELISA method can be used for the initial examination of HIV for unpaid blood donation. Specimens with uncertain HIV antibodies can be diagnosed by combining nucleic acids and antibodies. The blood station staff should do a good job of blood donors' health consultations, and isolate HIV-infected persons as much as possible before blood donation to ensure blood safety.
作者
施克飞
SHI Ke-fei(Changzhou Central Blood Station, Liyang Sub-station, Liyang, Jiangsu Province, 213300 China)
出处
《世界复合医学》
2019年第7期52-54,共3页
World Journal of Complex Medicine
