摘要
目的:通过评估布鲁氏菌病患者脊柱炎和关节炎疼痛分值以及检测布鲁氏菌病患者血清中IFN-γ、TNF-α、IL-6、IL-4和IL-2的水平变化,初步探讨布鲁氏菌病患者疼痛症状与血清中Th1/Th2细胞因子的关系。方法:收集307例(其中104例脊柱炎、133例关节炎、70例既有脊柱炎又有关节炎)布鲁氏菌病患者血标本作为病例组,同时收集100例健康人为对照组,用流式液相多重蛋白定量技术(CBA)检测IFN-γ、TNF-α、IL-6、IL-4和IL-2的变化。结果:与正常对照组相比,布病合并关节炎和脊柱炎患者中IFN-γ和IL-2的水平明显降低,差异具有统计学意义(P<0.01);当布病患者疼痛分值改变时,IFN-γ和IL-2的水平无明显变化,差异没有统计学意义(P>0.05);而布病合并关节炎和脊柱炎患者中TNF-α、IL-6和IL-4的水平增高,且患者疼痛分值越高,TNF-α、IL-6和IL-4的水平也越高,差异具有统计学意义(P<0.01)。结论:布鲁氏菌病患者疼痛分值越高,Th2细胞因子(IL-4)的免疫抑制功能相对增强,细胞因子TNF-α和IL-6的炎性反应越高。
Objective: To investigate the relationship between pain symptoms and serum Th1/Th2 cytokines through detecting IFN-γ、TNF-α、IL-6、IL-4 and IL-2 levels in patients with brucellosis. Methods: This study collected 224 patients with brucellosis(including 158 cases of spondylitis,184 cases of arthritis,70 cases of spondylitis and arthritis) as a case group.At the same time,100 healthy people were collected as control group,the changes of IFN-γ,TNF-α,IL-6,IL-4 and IL-2 were measured by CBA method. Results: Compared with control,the levels of IFN-γ and IL-2 were significantly lower in arthritis and spondylitis complications of brucellosis ( P <0.01),when the pain scores of patients with brucellosis changed,the levels of IFN-γ and IL-2 was no significant change ( P >0.05).The levels of TNF-α,IL-6,and IL-4 were increased,and the higher the pain score,the higher the levels of TNF-α,IL-6 and IL-4 ( P <0.01). Conclusion: The higher the pain score of patients with brucellosis,the higher the immunosuppressive function of Th2 cytokine (IL-4),and the higher the inflammatory response of cytokines TNF-α and IL-6.
作者
庞盼
张甜
关建萍
张峰波
扈会整
彭巧君
PANG Pan;ZHANG Tian;GUAN Jian-Ping;ZHANG Feng-Bo;HU Hui-Zheng;PENG Qiao-Jun(Department of Clinical Laboratory,the 215 Hospital of Shaanxi Nuclear Industry,Xianyang 712000,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2019年第15期1880-1886,共7页
Chinese Journal of Immunology
基金
新疆维吾尔自治区传染病医院2017年度院内科研课题(QLY-KY-2017003)
