摘要
以杜仲叶为原料,柠檬酸-磷酸二钠缓冲溶液作为提取溶剂,探索了酶法、超声波辅助酶法、超声波辅助半仿生法对京尼平苷的提取率,筛选出杜仲叶中京尼平苷的最佳提取方法。利用正交实验和单因素实验,考察了温度、时间、料液比以及缓冲液的pH对提取率的影响。实验结果表明,超声波辅助半仿生法的提取率最高。当提取温度为65℃、料液比为1∶40、处理时间为50min时,杜仲叶中京尼平苷的提取率高且稳定性好,提取率达到31.3%。
The optimal extraction method of geniposide in Eucommia ulmoides leaves was studied by enzymatic extraction, ultrasonic- assisted enzymatic, and ultrasonic assisted semi-bionic method applied geniposide as extraction solvent. The effects of temperature, time, pH buffer and solid-liquid ratio on the extraction rate were investigated by orthogonal experiments and single factor tests. The results showed that the extraction rate of ultrasonic assisted semi-bionic was highest when the extraction temperature was 65℃, the ratio of solid-liquid was 1:40(g:mL), treatment time was 50 min, the extraction rate of geniposide in Eucommia ulmoides leaves was high with 31.3% and highly stability.
作者
邓佑林
陆婷婷
杨盼盼
王美春
唐石
DENG Youlin;LU Tingting;YANG Panpan;WANG Meichun;TANG Shi(College of Chemistry and Biological Engineering, Hechi University, Yizhou 546300, China;College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China)
出处
《化工技术与开发》
CAS
2019年第8期6-10,16,共6页
Technology & Development of Chemical Industry
基金
国家自然科学基金项目（21662013）
广西高校微生物及植物资源开发利用重点实验室开放课题（2018HL006）
关键词
杜仲叶
京尼平苷
正交试验
酶法
超声波辅助法
Eucommia ulmoides leaves
geniposidic
orthogonal test
enzyme method
ultrasonic-assisted method
