摘要
目的采用HPLC法测定杜仲降压片中绿原酸、迷迭香酸、黄芩苷的的含量。方法采用Waters Sunfire C18色谱柱(250 mm×4. 6 mm,5μm),流动相为乙腈(A)-0. 2%磷酸(B),梯度洗脱(0~12 min、10%A,12~13 min、10%~25%A,13~28 min、25%A),检测波长分别为327(0~24 min)、316 nm(24~28 min),流速1. 0 m L·min^-1,柱温35℃。结果绿原酸、迷迭香酸、黄芩苷的线性范围分别为1. 01~16. 16、2. 57~41. 21、19. 46~311. 36μg·m L^-1;平均加样回收率分别为96. 96%、98. 73%、99. 05%,RSD分别为0. 86%、1. 11%、1. 05%(n=9)。结论所用方法简便可行、重复性好,可用于杜仲降压片中3种有效成分的含量测定,为杜仲降压片的质量控制提供了依据。
OBJECTIVE To establish an HPLC method for the determination of chlorogenic acid, rosmarinic acid and baicalin in Duzhong jiangya tablet. METHODS The determination of chlorogenic acid, rosmarinic acid and baicalin was executed on a Waters Sunfire C18 column ( 250 mm× 4. 6 mm,5 μm ), where the mobile phase consisted of acetonitrile (A ) and 0. 2% phosphoric acid ( B) with gradient elution(10% A at 0 -12 min, 10%-25% at 12 - 13 min,25% A at 13 -28 min) at a flow rate of 1.0 mL ·min^-1.The column temperaturewasset at 35℃,and the detection wavelengths were 327 nm at 0 - 24 min and 316 nm at 24 -28 min. RESULTS A good linearity of chlorogenic acid, rosmarinic acid and baicalin was obtained in the range of 1.01-16. 16μg·mL^-1 ,2. 57 -41.21 μg·mL^-1 and 19. 46 - 311. 36 μg·mL^-1 , respectively. The average recovery for chlorogenic acid, rosmarinic acid and baicalin was 96. 96%(RSD=0.86%),98.73%(RSD = 1.11%) and 99.05%(RSD =1.05%), respectively. CONCLUSION The method is simple, feasible and repeatable, and can be used for the quality control of Duzhong jiangya tablet.
作者
何艳
胡小祥
张霞
张辉
HE Yan;HU Xiaoxiang;ZHANG Xia;ZHANG Hui(School of Pharmacy,Xiangnan University,Chenzhou,Hunan ,423000 P. R. China;Chenzhou Institute Center for Food and Drug Control,Chenzhou,Hunan ,423000 P. R. China)
出处
《华西药学杂志》
CAS
CSCD
2019年第4期410-412,共3页
West China Journal of Pharmaceutical Sciences
基金
郴州市2017年科技计划项目(cxy2017023)
湖南省食品药品安全科技项目(湘食药科R201810)
湖南省“十三五”药学应用特色学科基金(湘教通[2018]469号)
关键词
杜仲降压片
高效液相色谱法
变换波长
绿原酸
迷迭香酸
黄芩苷
含量测定
质量控制
Duzhong jiangya tablet
HPLC
Variable wavelength
Chlorogenic acid
Rosmarinic acid
Baicalin
Content determination
Qualitiy control
