摘要
目的测定银黄含片中绿原酸和黄芩苷的含量.方法采用反相高效液相色谱法,DiamonsilTMC18柱,甲醇-水-磷酸(体积比52:48:0.1)为流动相,流速为0.9 mL·min-1;检测波长324 nm;柱温为室温.结果绿原酸在1.50～45.00μg·mL-1浓度范围内呈良好的线性关系,平均回收率为98.1%,方法精密度(RSD)为0.38%(n=5).黄芩苷在45.00～135.0μg·mL-1浓度范围内呈良好的线性关系,平均回收率为98.9%,方法精密度(RSD)为0.14%(n=5).结论该法简便、准确,能有效地控制该制剂的质量.
Objective To determine the contents of chlorogenic acid and baicalin in Yinhuang buccal tablets. Methods RP-HPLC method was performed on a Diamonsi^TM C18 column with UV detector at 324 nm. The mobile phase was methanol -water-phosphoric acid （52： 48：0.1 ）, pumped at a flow rate of 0.9 ml/min. Results The standard curves were linear from 1.50 to 45.00 mg/ml for chlorogenic acid and from 45.00 to 135.0 mg/ml for baicalin. The average recoveries were 98.5% for chlorogenic acid and 99.0% for baicalin. RSD was O. 38 % for chlorogenic acid （ n = 5 ） and 0. 14 % for baicalin （ n = 5 ）. Conclusions The method is simple, accurate and reliable for quality control of Yinhuang buccal tablets.
出处
《广东药学院学报》
CAS
2005年第6期683-684,共2页
Academic Journal of Guangdong College of Pharmacy
