摘要
目的探究阿萨伊对虚热、虚寒证小鼠的生物学效应的影响及验证阿萨伊性凉的药性假说。方法将昆明种雄性小鼠分为空白组、虚热模型组、虚热模型+生地黄组、虚热模型+阿萨伊组、虚寒模型组、虚寒模型+肉桂组、虚寒模型+阿萨伊组,以生地和肉桂做为对照药,采用每日灌胃给予甲状腺片溶液、氢化可的松溶液1次,连续14天的方法制备小鼠虚热、虚寒模型;采用冷热板示差法探讨了阿萨伊对小鼠温度趋向性的干预作用;通过放免法检测环核苷酸、甲状腺激素等生化指标变化。结果阿萨伊同生地黄一致,能改善甲状腺组小鼠的虚热状态,能够增加虚热小鼠体重,能使虚热小鼠在低温区停留比例明显下降( P <0.001),能使环磷酸腺苷(cyclic adenosine monophosphate,cAMP)、环磷酸鸟苷(cyclic guanosine monophosphate,cGMP)、cAMP/cGMP值明显下降( P <0.05 , P <0.05, P <0.001),也能使三碘甲腺原氨酸(triiodothyronine,T3)、甲状腺素(thyroxine,T4)水平有显著下降( P <0.001, P <0.01),表现出对虚热模型小鼠的调整作用;阿萨伊与肉桂相反,对虚寒模型小鼠的指标纠正不明显,仅能使T4含量明显上升( P <0.01)。结论本文采用同类比较、异类反证的方法证明了阿萨伊的中药药性为寒凉。
Objective To explore the effects of A■aí on the biological effects on deficiency-heat and deficiency-cold syndrome in mice and to test the hypothesized cold nature of A■aí. Methods Kunming male mice were randomly divided into blank group, deficiency-heat model group, deficiency-heat + Radix Rehmanniae Recens group, deficiency-heat + A■aí group, deficiency-cold model group, deficiency-cold + Cortex Cinnamomi group, deficiency-cold + A■aí group . The mice model of deficiency-heat and deficiency-cold were prepared by oral administration of thyroid tablet solution and hydrocortisone solution once a day for 14 days with Radix Rehmanniae Recens and Cortex Cinnamomi as control drugs. Deficiency-heat model group and deficiency-cold model group were prepared by continuous 14- day method. The intervention effects of Acai on temperature tropism in mice were investigated by using cold-hot plate differential method. Cyclic nucleotides, thyroid hormones and other biochemical indicators were detected by radioimmunoass. Results A■aí showed the same effects of Radix Rehmanniae Recens in improving the deficiency-heat state of mice in thyroid group, and it can increase the weight of mice with deficiency-heat. A■aí can decrease the percentage of mice with deficiency-heat in low temperature significantly ( P <0.001), decreasing the values of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) and cAMP/cGMP ( P <0.05, P <0.05, P <0.001), and decreasing the levels of triiodothyronine (T3) and thyroxine (T4)( P <0.001 , P <0.01);Contrary to Cortex Cinnamomi , the index correction of deficiency-cold model mice was not obvious, and it only increased the content of T4 significantly ( P <0.01). Conclusion This paper proves that traditional Chinese medicine in A aí is cold by a comparative pharmacological method.
作者
周雪
乐娜
王玉杰
赵子楠
瞿研
王林元
张建军
ZHOU Xue;YUE Na;WANG Yujie(School of Chinese Medicine,Beijing Universityof Chinese Medicine,Beijing 100029,China)
出处
《环球中医药》
CAS
2019年第8期1172-1176,共5页
Global Traditional Chinese Medicine
基金
北京中医药大学中青年教师自主课题(2018-JYBZZ-JS028、2016-JYB-JSPY-013)
北京中医药大学在读研究生项目(2015-JYB-XS002)
国家自然科学基金(81173569)
关键词
阿萨伊
虚热证
虚寒证
冷热板示差法
生物学表达特征
A aí
Deficiency-heat syndrome
Deficiency-coldsyndrome
Cold-hot plate differential method
Biologicalexpression characteristics
