摘要
目的探索中国与欧洲沿海发达地区婴幼儿肠道菌群的结构差异及随年龄增长的变化趋势。方法招募深圳地区健康婴幼儿35名并收集其粪便样本,采用Illumina的MiSeq平台对肠道菌群的16S rRNA V4序列进行测序。同时,结合40名芬兰艾斯堡地区健康婴幼儿肠道菌群数据,进行两地儿童菌群结构比较。结果中国与芬兰地区幼儿肠道菌群结构较为一致,在物种多样性及均匀度方面差异无统计学意义。菌属水平分析中,两组幼儿肠道内均呈现以拟杆菌属、双歧杆菌属为主导的菌群结构。回归分析表明,幼儿肠道内菌群多样性随年龄的增长明显增加(R^2=0.174,P<0.05),0~1岁与1~3岁幼儿的菌群结构差异有统计学意义(R^2=0.079,P<0.05)。韦荣球菌、肠球菌和布劳特菌等菌属随年龄增长丰度明显降低,而粪杆菌、瘤胃球菌、罗斯菌等菌属显著增加。结论生命早期肠道菌群的发育主要与年龄因素密切相关,地区因素为次要因素。
Objective To explore the structural differences in gut microbiota between infants from Chinese and European developed coastal cities,and the changing trend of microbial community along with age.Methods We enrolled a total of 35 healthy infants at Shenzhen and sampled fecal specimens.Bacterial 16S rRNA V4 region was sequenced using Illumina MiSeq platform.We also collected the fecal microbiota data of 40 healthy infants in Finland to conduct comparison analysis.Results The intestinal microbiota of infants from China and Finland were relatively consistent,and the differences in bacterial diversity and evenness were not statistically significant.At the genus level,Bacteroides and Bifidobacteria were the dominated genera.Regression analysis revealed that the diversity of intestinal microbiota increased significantly along with age(R^2=0.174,P<0.05),and the differences in intestinal microbiota between infants aged 0-1 year and 1-3 years were also significant(R^2=0.079,P<0.05).The abundances of Veillonella,Enterococcus and Brauterella decreased during development,whereas those of Faecalibacterium,Ruminococcus and Rothia increased gradually.Conclusion Age is mainly associated with the development of intestinal microflora in early life,and region acts as the secondary influence factor.
作者
谢淦
王艳芬
李嘉琪
郑跃杰
周乐天
杨琴
戴文魁
禹定乐
XIE Gan;WANG Yanfeng;LI Jiaqi;ZHENG Yuejie;ZHOU Letian;YANG Qin;DAI Wenkui;YU Dingle(Shenzhen Children's Hospital,Shenzhen,Guangdong 518038,China)
出处
《中国微生态学杂志》
CAS
CSCD
2019年第7期772-775,共4页
Chinese Journal of Microecology
关键词
婴幼儿
肠道菌群
高通量测序
Infants
Gut microbiota
High throughput sequencing
