摘要
目的:建立全反式维甲酸(ATRA)诱导分化的NB4细胞浸润裸鼠肺组织模型,为探讨诱导分化综合征(DS)的发生机制、预防和治疗提供研究平台。方法:首先,取对数生长期的NB4细胞在ATRA诱导下、在RPMI 1640培养基中、在37℃和5%CO2的条件下培养,72 h后收获诱导分化后的NB4细胞接种裸鼠尾静脉。然后,30天后处死裸鼠取肺组织,用G显带方法检测裸鼠肺组织的染色体核型,HE染色观察裸鼠肺组织学,电子显微镜观察超微结构,RT-PCR法和FISH技术检测裸鼠肺组织PM L-RARa m RNA转录本及PML-RARa基因的表达。结果:实验组染色体核型符合NB4细胞特征,在组织学和超微结构上明显见到分化的NB4细胞浸润裸鼠肺组织,检测到PM L-RARa m RNA转录本和PML/RARa融合基因;对照组未见到分化的NB4细胞浸润裸鼠肺组织,PML-RARa m RNA转录本和PML/RARa融合基因检测阴性。结论:用ATRA诱导分化的NB4细胞成功的建立了浸润裸鼠肺组织模型,为探讨诱导分化综合征(DS)的发生机制、预防和治疗提供了研究平台。
Objective: To establish a nude mice model of differentiated NB4 infiltration into the lung tissue, and provide approach for the study on differentiation syndrome(DS). Methods: Firstly, logarithmic growth NB4 cells in RPMI 1640 medium containing 0.5μmol/L ATRA were cultured at 37℃ in a humidified 5% CO2 incubation and were induced differentiation by ATRA. Then the cells induced differentiation by ATRA were harverted at 72 h after incubation and were injected into the tail vein of BALB/c-nu female nude mice(SPF level, 6 weeks old). Then, after 30 days, the rats were sacrificed, the pathology, ultructure and chromosome karyotype of rats lung tissue were examined, PML-RARa m RNA and PML-RARa gene of rats lung tissue were measured by RT-PCR and by FISH. Results: In the trial group, the chromosome karyotype of lung tissue was the same with the NB4 cells specialized by ATRA. In the trial groups, the infiltration of NB4 cells specialized by ATRA into the lung tissue was observed by HE staining and electron microscope, but not in the control group. In the trial group, the PM L-RARa m RNA and PML-RARa gene expressed in the lung tissue, but not in the control group.Conclusions: A nude mice model of BN4 differentiated by ATRA infiltration into the lung tissue was established, which may contribute to the study on the pathogenesis, prevention and treatment of DS.
作者
胡龙虎
崔喆
原冬伟
孔德胜
贾垂明
马琳娜
吴闯
王雪峰
韩辉
HU Long-hu;CUI Zhe;YUAN Dong-wei;KONG De-sheng;JIA Chui-ming;MA Lin-na;WU Chuang;WANG Xue-feng;HAN Hui(The First Affiliated Hospital,Harbin Medical University,Harbin,Heilongjiang,150001,China;Harbin institute of veterinary medicine,Chinese academy of agricultural sciences,Harbin,Heilongjiang,150001,China;The Fourth Affiliated Hospital,Harbin Medical University,Harbin,Heilongjiang,150001,China;The Third Affiliated Hospital,Harbin Medical University,Harbin,Heilongjiang,150081,China;The Fourth Hospital of Harbin,Harbin,Heilongjiang,150026,China;Qing Longshan Psychiatiric Hospital of Nanjing,Nanjing,Jiangshu,210000,China)
出处
《现代生物医学进展》
CAS
2019年第11期2025-2031,2006,共8页
Progress in Modern Biomedicine
基金
黑龙江省教育厅科研基金项目(12511334)
哈尔滨医科大学附属第一医院博士启动基金
