摘要
背景:研究证实深层海水可通过激活AMPK通路下调小鼠对糖的摄入,进而有效缓解高脂肪饮食诱导的小鼠糖尿病进程,然而目前有关深层海水在糖尿病创伤难愈合中的作用研究鲜有报道。目的:探讨深层海水对糖尿病小鼠创面的修复作用机制。方法:取健康昆明种雄性小鼠48只(购于昆明医科大学实验动物中心),通过腹腔注射链脲佐菌素建立糖尿病模型;饲养14 d后建立糖尿病小鼠背部创面模型,随机分4组处理,其中3组分别喂食纯净水(对照组)、自来水(自来水组)、深层海水(深层海水组),抑制剂组喂食深层海水的同时股静脉注射Wnt/β-catenin抑制剂XAV-939。创面造模后第3,7,10天,检测对照组、自来水组、深层海水组小鼠血糖和体质量、创面愈合及创面组织中β-catenin、GSK-3β和Rspo-3蛋白表达,观察对照组、深层海水组、抑制剂组创面愈合率、创面组织形态变化及血清中丙二醛、超氧化物歧化酶和谷胱甘肽过氧化物酶水平。实验获得中国科学院昆明动物研究所实验动物伦理委员会批准(批准号:KPRC-2017091)。结果与结论:(1)深层海水对糖尿病小鼠小鼠体质量和血糖值无影响;(2)深层海水组不同时间点的创面愈合率明显高于自来水组和对照组(P均<0.01);(3)Western blotting检测显示与对照组和自来水组比较,深层海水组创面组织中β-catenin、Rspo-3蛋白表达升高,GSK-3β蛋白表达下降;(4)苏木精-伊红染色显示,与对照组比较,深层海水组新生肉芽组织中血管内皮细胞和新生毛细血管增多,炎性细胞浸润较少,成纤维细胞增生明显;抑制剂组与对照组病理表现无明显差异(P> 0.05);深层海水组不同时间点的创面愈合率明显高于自来水组和抑制剂组(P均<0.01);(5)与对照组比较,深层海水组超氧化物歧化酶、谷胱甘肽过氧化物酶活性升高(P <0.01,P <0.001),丙二醛浓度下降(P <0.05,P <0.01);抑制剂组3指标水平与�
BACKGROUND: Deep seawater has been shown to effectively alleviate the process of diabetes in mice induced by high fat diet through activating AMPK pathway, but there are few studies about the role of deep seawater in the wound healing of diabetes mellitus.OBJECTIVE: To explore the mechanism of deep seawater on the cutaneous wound healing in diabetic mice.METHODS: Forty-eight healthy male Kunming mice(provide by Laboratory Animal Center of Kunming Medical University) were selected and were given intraperitoneal injection of streptozotocin to establish diabetic model. After 14 days of incubation, the back would model was established, and the mouse models were randomly divided into four groups: control group(purified water), tap water group, deep seawater group and inhibitor group(deep seawater + venous injection of Wnt/β-catenin inhibitor, XAV-939). Subsequently, the blood glucose and body mass of model mice were observed at 3, 7 and 10 days after wound modeling, and the healing of the wound was observed. The morphological changes of the wound were evaluated. The expression levels of β-catenin, GSK-3β and Rspo-3, malondialdehyde, superoxide dismutase and glutathione peroxidase in the serum were detected. The study was approved by the Laboratory Animal Ethics Committee of Kunming Institute of Zoology, China Academy of Sciences, approval No. KPRC-2017091.RESULTS AND CONCLUSION:(1) Deep seawater showed no significant effect on the body mass and blood glucose.(2) The wound healing rate in the deep seawater group was significantly higher than that in the tap water and control groups at different time points(all P < 0.01).(3)Western blot results showed that the expression levels of β-catenin and Rspo-3 were higher in the deep seawater group than the control and tap water groups, but GSK-3β was down-regulated.(4) Hematoxylin-eosin staining showed that compared with the control group, vascular endothelial cells, new capillaries and fibroblast cells proliferation in the deep seawater group were increased, but the numbe
作者
李为明
孙大力
李奕俊
李树民
孙岩波
崔进
徐鹏远
许青文
Li Weiming;Sun Dali;Li Yijun;Li Shumin;Sun Yanbo;Cui Jin;Xu Pengyuan;Xu Qingwen(Second Ward of Department of GastrointestinalSurgery,the Second Affiliated Hospital of Kunming Medical University,Kunming 650101,Yunnan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第31期5017-5022,共6页
Chinese Journal of Tissue Engineering Research
基金
云南省应用基础研究联合专项(2015FB057),项目负责人:李为明
云南省外科临床营养研究中心子课题(2016NS272),项目负责人:李为明~~
