摘要
目的观察补气生肌方对糖尿病大鼠难愈创面的血管生成和促进血管生成的因子表达的影响。方法选取48只雄性SD大鼠,经高脂高糖饲料喂养1周后,按35 mg/kg腹腔注射1%链脲佐菌素(STZ)溶液,糖尿病模型造模成功后制作创面模型,随机分为观察组(24只)和对照组(24只),其中观察组局部应用补气生肌中药涂抹,对照组应用凡士林涂抹,均为每日1次。采用免疫荧光法检测血管生成情况,PCR检测创面组织中血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)和碱性成纤维细胞生长因子(Basic fibroblast growth factor,b FGF)的基因表达。结果观察组第3天、第7天、第14天的创面愈合率高于对照组(P<0.05)。免疫荧光检测结果显示,第7天观察组、对照组的创面组织CD-34阳性细胞数分别为34.39%±3.46%和12.42%±1.53%,两组比较差异有统计学意义(P<0.05)。PCR检测结果显示,在第3天、第7天、第14天,观察组创面组织VEGF-A、b FGF m RNA表达高于对照组(P<0.05)。结论补气生肌方在治疗糖尿病难愈性创面时,可上调VEGF-A和b FGF基因表达,促进创面组织血管生成,有利于难愈性创面的愈合和重建。
Objective To observe the influence of Buqi Shengji formula on angiogenesis of diabetic refractory w ounds and vascular endothelial grow th factor. Methods SD male rats（ n = 48） w ere given intraperitoneal injection of 1% STZ 35 mg/kg after being fed w ith high-fat and high-sugar diet,and skin w as scratched to establish the diabetic w ound model after the diabetic model w as established. The model rats w ere randomly divided into 2 groups. Observation group（ n = 24） w as given Buqi Shengji formula,w hile control group（ n = 24） w as given vaseline. The microvessel count in granulation tissue w as determined by immunofluorescence,and the expression of VEGF and b FGF w ere determined by PCR. Results The w ound healing rate in observation group w as higher than that of control group on the3 rd,7 th and 14 th day（ P〈0. 05）. The immunofluorescence results show ed that the number of CD-34 positive cells w as34. 39% ± 3. 46% in observation group and 12. 42% ± 1. 53% in control group respectively,and there w as significant difference betw een the tw o groups（ P〈0. 05）. The expression of VEGF-A and b FGF m RNA in the w ound tissue of observation group w as higher than that of control group on the 3 rd,7 th and 14 th day（ P〈0. 05）. Conclusion Buqi Shengji formula can up-regulate the expression of VEGF-A and b FGF gene in the treatment of diabetic refractory w ounds,and promote the angiogenesis of w ound tissue w hich is beneficial to the healing and reconstruction of refractory w ounds.
作者
陈玉姣
白晓苏
曾丽红
CHEN Yu-jiao BAI Xiao-su ZENG Li-hong(the Third Department of Internal Medicine Department of Endocrinology Department of Neurology, Longhua Area People＇s Hospital of Shenzhen City, Shenzhen 518000, China)
出处
《实用药物与临床》
CAS
2017年第10期1127-1130,共4页
Practical Pharmacy and Clinical Remedies
关键词
补气生肌方
糖尿病
创面
血管
Buqi Shengji formula
Diabetes
Wound
Blood vessels
