摘要
目的:探讨趋化素样因子超家族6(CMTM6)、程序性死亡受体-配体1(PD-L1)在脑胶质瘤组织中的表达及其与患者临床病理特征的相关性。方法:选用2012年1月至2015年12月于郑州大学第五附属医院神经外科手术切除的86例脑胶质瘤标本及30例头颅外伤去骨瓣减压患者的脑组织标本(对照组),用免疫组化法、WB法检测脑胶质瘤组织中CMTM6和PD-L1蛋白的分布与表达,用两独立样本t检验分析CMTM6与PD-L1的表达差异,用单因素χ^2检验分析CMTM6和PD-L1表达与患者临床病理特征之间的关系。结果:胶质瘤组织中CMTM6的表达水平明显高于对照组织(P<0.01),高病理级别(WHOⅢ-Ⅳ)胶质瘤组织中CMTM6和PD-L1的表达水平均明显高于低病理级别(WHOⅠ-Ⅱ)胶质瘤组织(均P<0.01)。CMTM6表达与患者脑胶质瘤病理级别、头晕病史、癫痫发作、PD-L1表达有关(均P<0.05),PD-L1表达与脑胶质瘤病理级别、癫痫发作、CMTM6表达有关(均P<0.05)。结论:CMTM6和PD-L1在脑胶质瘤组织中均高表达且两者间有一定相关性,有望用于研究脑胶质瘤信号通路。
Objective:To investigate the expressions of chemokine-like factor superfamily 6(CMTM6)and programmed cell death ligand 1(PD-L1)in glioma tissues and their correlation with clinicopathological features of patients.Methods:From January 2012 to December 2015,86 brain glioma tissues and 30 brain tissues(Control group)from patients operated with decompressive of craniotomy were collected from the Fifth Affiliated Hospital of Zhengzhou University.The distribution and expressions of CMTM6 and PD-L1 protein in brain glioma tissues were detected by immunohistochemistry and WB methods.The differential expression of CMTM6 and PDL1 between glioma tissues and normal brain tissues was analyzed by t test of two independent samples.Single variantχ^2 test was used to analyze the relationship between the expression of CMTM6,PD-L1 and the clinicopathological features of patients.Results:The expression of CMTM6 in glioma tissues was significantly higher than that in control tissues(P<0.01).The expression levels of CMTM6 and PD-L1 in high pathological grade(WHO III-IV)glioma tissues were significantly higher than those in low pathological grade(WHO I-II)glioma tissues(all P<0.01).The expression of CMTM6 was correlated with pathological grade,dizziness history,epilepsy seizure and PD-L1 expression(all P<0.05),while the expression of PD-L1 was correlated with pathological grade,epilepsy seizure and CMTM6 expression(all P<0.05).Conclusion:There is a correlation between the expression of CMTM6 and PD-L1 in glioma tissues,both of which are highly expressed and are expected to be used to study glioma signaling pathways.
作者
杨卓
王新军
张强
付旭东
YANG Zhuo;WANG Xinjun;ZHANG Qiang;FU Xudong(Department of Neurosurgery,the Fifth Affiliated Hospital of ZhengzhouUniversity,Zhengzhou 450052,Henan,China;Department of Radiation Oncology and Comprehensive Cancer Center,Universityof Michigan,Michigan 48109,USA)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2019年第7期782-787,共6页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(No.81874068)
河南省重点专科服务能力提升计划工程资助项目(No.2017-56)~~
关键词
趋化素样因子超家族6
程序性死亡受体-配体1
脑胶质瘤
病理参数
信号通路
chemokine-like factor superfamily 6(CMTM6)
programmed cell death ligand 1(PD-L1)
gliomas
pathological parameters
signaling pathway
