摘要
该研究以从土壤中分离筛选得到的一株高产高纯度β-胡萝卜素的菌株S3-1为研究对象,采用分子生物学技术对其进行鉴定,并通过单因素及正交试验对其发酵工艺进行优化。结果表明,菌株S3-1被鉴定为近玫色锁掷孢酵母(Sporidiobolus pararoseus)。最优发酵工艺为葡萄糖40g/L、酵母浸粉30g/L、硫酸镁0.2g/L、初始pH值5.0。在此最优发酵工艺条件下,β-胡萝卜素产量最高,为(3.08±0.46)mg/L,纯度为(60.01±2.66)%,为工业化发酵生产高纯度β-胡萝卜素提供工艺参考。
Using the strain S3-1 with high yield and high purity of β-carotene isolated from soil as research object,the strain was identified by molecular biological technique,and its fermentation process was optimized by single factor and orthogonal experiments. The results showed that the strain S3-1 was identified as Sporidiobolus pararoseus. The optimal fermentation process was glucose 40 g/L,yeast extract 30 g/L,MgSO4 0.2 g/L,and initial pH 5.0. Under the optimal fermentation conditions,the yield of β-carotene was (3.08±0.46) mg/L,and the purity was (60.01±2.66)%,which provided a process reference for the industrial fermentation to produce high purity β-carotene.
作者
丁长河
张梦涵
DING Changhe;ZHANG Menghan(Key Laboratory of Natural Pigment Preparation,Henan Province,College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China)
出处
《中国酿造》
CAS
北大核心
2019年第7期116-121,共6页
China Brewing
关键词
Β-胡萝卜素
菌种鉴定
发酵工艺
优化
β-carotene
strain identification
fermentation process
optimization
