摘要
本文以CTAB为包被试剂合成了球形、棒状和三角形三种不同形态的纳米金颗粒,研究了它们对金黄色葡萄球菌(Staphylococcus aureus,S.aureus)和大肠杆菌(Escherichia coli,E.coli)杀菌性能的差异.通过平板计数法和浊度法;确定了它们对两种菌的最小杀菌浓度(minimum bactericidal concentrations,MBC)和最小抑菌浓度(minimum inhibitory concentrations,MIC).棒状纳米金、三角形纳米金、球形纳米金对大肠杆菌的MIC分别为0.65μg/mL、3.71μg/mL、21.21μg/mL,MBC分别为1.30μg/mL、11.09μg/mL、21.21μg/mL;对金黄色葡萄球菌的MIC分别为0.26μg/mL、0.56μg/mL、2.65μg/mL,MBC分别为0.52μg/mL、1.11μg/mL、2.65μg/mL.结果表明无论是对大肠杆菌还是金黄色葡萄球菌,棒状纳米金的杀菌效果均高于其它两种形态,并且三种不同形态的纳米金对金黄色葡萄球菌的杀菌效果均优于大肠杆菌.
In this work,the rod,triangular and spherical gold nanoparticles were synthesized using cetyltrimethyl ammonium bromide as modified agent.The antibacterial properties of three different forms against the S.aureus and the E.coli were respectively studied.The minimum bactericidal concentration and minimum inhibitory concentration to bacteria were determined by plate counting and turbidity.For E.coli ,the MBC of the rod,triangular and spherical gold nanoparticles was 1.30 μg/mL,11.09 μg/mL and 21.21 μg/mL,the MIC of the rod,triangular and spherical gold nanoparticles was 0.65 μg/mL,3.70 μg/mL and 21.21 μg/mL.For S.aureus ,the MBC of the rod,triangular and spherical gold nanoparticles was 0.52 μg/mL,1.11 μg/mL and 5.30 μg/mL,and the MIC of the rod,triangular and spherical gold nanoparticles was 0.26 μg/mL,0.56 μg/mL and 2.65 μg/mL.The antimicrobial activity of the rod gold nanoparticles is superior to other two types of gold nanoparticles.And no matter what shape of the gold nanoparticles,the antimicrobial activity to S.aureus is better than that to E.coli.
作者
马亚云
刘梅
李娇
李宣仪
杨宗琪
MA Ya-yun;LIU Mei;LI Jiao;LI Xuan-yi;YANG Zong-qi(School of Food Engineering and Nutritional Science,Shanxi Normal University,Xi’an 710119,China)
出处
《聊城大学学报（自然科学版）》
2019年第5期64-70,共7页
Journal of Liaocheng University:Natural Science Edition
基金
中央高校基本科研业务费资助项目(GK201802012)
陕西省科技计划项目(2017NY-121)资助
关键词
纳米金
不同形态
金黄色葡萄球菌
大肠杆菌
抗菌性能
AuNPs
different shape
Staphylococcus aureus
Escherichia coli
antimicrobial activity
