摘要
目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T和A1298C位点多态性与原发性男性不育症的关系。方法选取2018年1~7月在台州市中心医院就诊的的原发性男性不育患者104例为研究对象,选取同期健康查体的已育男性108例为对照组。采用荧光定量PCR技术检测104例男性不育症患者和108例健康已育男性的MTHFRC677T和A1298C位点的多态性,统计学分析不同基因型男性与原发性不育的相关性。结果原发性男性不育组和健康对照组MTHFRC677T位点TT基因型频率分别为23.08%、12.96%,T等位基因频率分别为43.75%、33.33%,两组间基因型频率和基因频率均存在显著性差异(χ^2=6.975,χ^2=4.859,P<0.05);原发性男性不育组和健康对照组的MTHFRA1298C位点CC基因型频率和C等位基因频率均无显著性差异(χ^2=0.108,χ^2=0.170,P>0.05)。结论MTHFR基因677位点C-T变异与原发性男性不育具有相关性,1298位点多态性与原发性男性不育无显著相关性。
Objective : To investigate the relationship between the polymorphisms of the C677T and A1298C locus of the methylenetetrahydrofolate reductase (MTHFR) gene and the primary male infertility. Methods : The C677T and A1298C polymorphisms were detected by using real-time fluorescent quantitative PCR in 104 male patients with primary infertility and 108 healthy males. Results : The frequencies of TT genotype of MTHFR C677T locus in the primary male infertility group and the healthy control group were 23.08% and 12.96% respectively,and the T allele frequencies were 43.75% and 33.33% respectively. There were significant differences in genotype frequencies and gene frequencies between the two groups(χ^2 =6.975,χ^2 =4.859, P <0.05). There was no significant difference in the frequency of CC genotype and the frequency of C allele in the MTHFR A1298C locus between the primary male infertility group and the health control group (χ^2 =0.108,χ^2 =0.170, P >0.05). Conclusions : The C-T mutation at 677 locus of MTHFR gene is correlated with male primary infertility. The polymorphism of 1298 locus is not significantly correlated with primary male infertility.
作者
徐佳佳
陈文举
梁玲芝
楼永良
XU Jia-jia;CHEN Wen-ju;LIANG Ling-zhi;LOU Yong-liang(School of Laboratory Medicine & Life Sciences,Wenzhou Medical University,Wenzhou 325000;Department of Clinical Laboratory,Taizhou Central Hospital,Taizhou 318000)
出处
《生殖医学杂志》
CAS
2019年第7期777-780,共4页
Journal of Reproductive Medicine
