摘要
目的基于多色流式方法,研究人类免疫缺陷病毒感染者与未感染的正常对照外周血自然杀伤细胞(naturalkillercell,NK)数目和亚群分布差异。方法用5种细胞表面抗体CD3、CD14、CD19、CD56、CD16及7-AAD建立NK细胞各亚群染色方案,并对20例人类免疫缺陷病毒男性感染者和20例男性正常对照外周血标本进行六色流式细胞术检测分析。结果人类免疫缺陷病毒感染者NK细胞数量(188±117.7)个/μL,低于正常对照组NK细胞(281.6±107.8)个/μL,差异有统计学意义(p<0.05);细胞毒性NK细胞百分比(64.8±20.0)%比正常对照细胞毒性NK细胞(87.9±5.5)%减少,差异有统计学意义(p<0.01);无功能型NK细胞百分比(24.1±19.4)%比正常对照细胞毒性NK细胞(5.0±3.2)%增多,差异有统计学意义(p<0.01)。以上改变与CD4^+T细胞计数、病毒载量均无相关性。结论免疫缺陷病毒感染引起NK细胞数量下降和亚群比例失调。
Objective To study the differences in the numbers and distributions of natural killer (NK) cell subsets in peripheral blood between human immunodeficiency virus (HIV) infected patients and healthy donors based on multi-color flow cytometry. Method Five cell-surface antibodies (CD3, CD14, CD19, CD56 and CD16) and 7-AAD were used to establish a multi-color flow cytometry staining protocol to detect NK cell subsets. Peripheral blood samples from 20 HIV-infected male patients and 20 healthy subjects were analyzed by six-color flow cytometry. Results The absolute number of NK cells in HIV- infected patients (188±117.7)/μL was significantly lower than that in healthy donors (281.6±107.8)/μL. The difference was statistically significant (p<0.05). The proportion of cytotoxic NK cells (64.8±20.0)% in HIV- infected patients was lower than that in healthy donors (87.9±5.5)% and the difference was statistically significant (p<0.01). The non-functional NK cell subset in HIV-infected patients (24.1±19.4)% was significantly higher than that in healthy donors (5.0±3.2)%. The difference was statistically significant (p<0.01). The above changes were not associated with CD4^+T cell counts and viral loads. Conclusion Decreased absolute number and the imbalanced subset distribution of NK cells were caused by HIV infection.
作者
曾永秦
杜娟
郝禹
李国力
逄晓莉
汪笛
樊立娜
李蓓
赵红心
Zeng Yongqin;Du Juan;Hao Yu;Li Guoli;Pang Xiaoli;Wang Di;Fan Lina;Li Bei;Zhao Hongxin(Infecttious Disease Department 1, Peking University Ditan Teaching Hospital, Beijing 100015, China;Reasearch Institute, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China)
出处
《国际病毒学杂志》
2019年第3期209-212,共4页
International Journal of Virology
基金
国家自然科学基金(81672000)
北京市科技新星计划(Z171100001117016)
首都临床特色应用研究(Z171100001017052).
