摘要
【目的】分析光系统Ⅱ PsbS基因在不同光照条件下,光系统Ⅱ PsbS基因的功能。【方法】利用转棉花PsbS基因的烟草(Nicotiana tabacum xanthin),T3代株系及非转基因(普通野生型,wt)烟草为材料,比较两者的生物学性状(叶面积、叶绿素含量)及在不同光强(75、240、450μmol/(m^2·s))下的生理(光合作用)性状。【结果】转基因植株前期叶面积增长较快,叶绿素组成成分的含量也存在差异,叶绿素a、b含量显著高于野生型烟草,转基因烟草具有更强的光合能力,并伴随着气孔导度(Gs)、蒸腾速率(Tr)提高。【结论】转PsbS基因可以提高烟草光合能力。
【Objective】To expound the function of Ⅱ PsbS gene in photo system from the point of view of photosynthesis according to the analysis of Ⅱ PsbS gene in photosystem under different light conditions.【Method】In this experiment,cotton PsbS transgenic( Nicotiana tabacum xanthin),T3 generation lines and non-transgenic( wild-type,wt) tobacco) were used as materials to compare the biological characters( leaf area,chlorophyll content) and physiological( photosynthetic) characters under different light intensities( 75,240 and 450 μmol/( m^2·s)) of the two types of tobaccos. 【Result】The leaf area of the transgenic plants increased rapidly in the early stage,and the content of chlorophyll components also varied. The contents of chlorophyll A and B were significantly higher than those of wild type tobacco,and the transgenic tobacco had stronger photosynthetic capacity accompanied by stomatal conductance( Gs),transpiration rate( Tr) increase. 【Conclusion】The transgenic PsbS gene can enhance the photosynthetic capacity of tobacco.
作者
曾晓燕
赵瑞海
李志博
魏亦农
ZENG Xiao-yan;ZHAO Rui-hai;LI Zhi-bo;WEI Yi-nong(Key Laboratory of Oasis Eco-agriculture of Xinjiang Production and Construction Corps,College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2019年第4期589-598,共10页
Xinjiang Agricultural Sciences
基金
国家自然科学基金“高温对棉花光合特性、叶绿素荧光及抗氧化体系的影响”(31560406)~~
关键词
烟草
PsbS基因
转基因
光合
tobacco
PsbS geng
overexpression
photosynthetic
