摘要
探究光照对‘冰川红叶’小檗(Berberis thunbergii ‘Bingchuanhongye’)生长和叶片呈色的影响,改善‘冰川红叶’的观赏效果,为其在城市园林建设中合理应用提供科学依据。本研究以盆栽‘冰川红叶’为研究对象,以‘紫叶’小檗(Berberis thunbergii ‘Atropurpurea’)为参照,采用遮荫网人工模拟4种光照环境(0%、30%、50%、90%遮荫)进行试验,对不同遮荫度下叶片颜色、表型性状、叶片反射光谱、光合生理参数等进行测定,并对‘冰川红叶’叶片进行叶绿体超微结构观测和转录组测序分析。结果表明:(1)随遮荫度增加, 2个品种叶色均表现出复绿现象,‘冰川红叶’叶色复绿更明显;叶绿素含量上升,类胡萝卜素、类黄酮、花色素苷含量及合成相关酶活性显著降低。(2)随遮荫度增加,‘冰川红叶’可溶性蛋白含量先下降后上升;可溶性糖含量、光系统Ⅱ最大光化学效率(Fv/Fm)先上升后下降,净光合速率(Pn)下降;遮荫环境下‘冰川红叶’叶片光谱特征反射率下降,红边参数指示其全光照环境下受到强光胁迫。(3)遮荫后根重比无显著变化,茎重比、叶面积、株高增长量先升后降,地径增长量显著降低;叶绿体内淀粉粒空洞,基质片层排列松散、肿胀,出现了质壁分离。(4)转录组学分析发现,各比较组中有181个共有差异表达基因(DEG);基因本体(GO)功能注释发现,其中较多基因与氧化还原过程、氧化还原酶活性相关。经京都基因与基因组百科全书(KEGG)显著性富集分析,筛选到类黄酮生物合成、类胡萝卜素生物合成等相关基因。通过与拟南芥(Arabidopsis thaliana)构建系统进化树,聚类到与类黄酮合成有关的R2R3-MYB家族S4、S5、S6、S7亚组的基因有7个,bHLH家族并未聚类到与类黄酮合成有关的亚组。进一步对候选转录因子与结构基因进行共表达网络构建,结果显示4个R2R3-MYB和2个bHLH影响类黄酮合成通路结�
To explore the effects of light on the growth and leaf color of Berberis thunbergii‘Bingchuanhongye’,improve its ornamental effect,and provide scientific basis for its rational application in urban garden construction,we took potted‘Bingchuanhongye’as research object and B.thunbergii‘Atropurpurea’as the control.The experiment was conducted by artificially simulating four light environments(0%,30%,50%and 90%of shading).The leaf color,phenotypic traits,leaf reflection spectrum,and photosynthetic physiological parameters of two varieties were examined,and chloroplast ultrastructural observation and transcriptome sequencing analysis of‘Bingchuanhongye’were performed.The results show:(1)with the increase of shading degree,the leaf color of two varieties turned green,and‘Bingchuanhongye’became more obvious.The chlorophyll content increased,the contents of carotenoids,flavonoids and anthocyanins and the activities of synthesis-related enzymes decreased significantly.(2)With the increase of shading degree,the content of soluble protein first decreased and then increased,soluble sugar content and maximum photochemical efficiency of photosystem II(Fv/Fm)first increased and then decreased,and net photosynthetic rate(Pn)decreased.The spectral reflectance of‘Bingchuanhongye’leaves decreased under the shade environment.The red edge parameter indicates high-light stress on‘Bingchuanhongye’under natural illumination.(3)Under different shading conditions,the root mass ratio was not significantly changed,the stem mass ratio,leaf area and plant height increment increased first and then decreased,while the ground diameter increment decreased significantly.Starch granules in chloroplasts became hollow,matrix lamellae were loosely arranged and swollen,and plasmolysis accured.(4)Transcriptomic analysis found that there are 181 shared DEGs in each comparison group.GO functional annotation found that more genes are related to redox processes and oxidoreductase activity.Some genes related to flavonoid biosynthe
作者
刘卫平
任亚超
杨敏生
王进茂
冀伟
张军
LIU Weiping;REN Yachao;YANG Minsheng;WANG Jinmao;JI Wei;ZHANG Jun(Forestry College,Hebei Agricultural University,Baoding,Hebei 071000,China;Hebei Key Laboratory for Tree Genetic Resources and Forest Protection,Baoding,Hebei 071000,China;Shijiazhuang Gaocheng District Ludu Municipal Gardening Engineering Co.Ltd.,Shijiazhuang 052160,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2023年第5期977-996,共20页
Plant Physiology Journal
基金
河北省重点研发计划(21326301D)。
关键词
‘冰川红叶’小檗
遮荫
叶色
光合生理
叶绿体超微结构
转录组分析
Berberis thunbergii‘Bingchuanhongye’
shading
leaf color
photosynthetic physiology
chloroplast ultrastructure
transcriptome analysis
