摘要
以4个品种转 Bt 基因抗虫棉及其对照常规棉为研究对象,采用ELISA试剂盒法检测其在不同生育期根际土壤 Bt 蛋白残留,同时比较速效养分含量的变化。结果表明:(1)棉花根际土壤中 Bt 蛋白的残留量在蕾期达到最大;在整个生育期4个品种转基因抗虫棉根际土壤中 Bt 蛋白的含量均显著高于对照常规棉( P <0.05),其中鲁棉研28号在蕾期显著高于对照常规棉1 029.66%,鲁棉研36号在蕾期显著高于对照常规棉2 573.45%,山农棉8号在苗期显著高于对照常规棉1 208.89%,鑫秋4号在花铃期显著高于对照常规棉793.18%。(2)棉花根际土壤中硝态氮含量在苗期最高,吐絮期最低,且在部分生育期转基因抗虫棉与对照常规棉差异显著;铵态氮含量在苗期最高,吐絮期最低,4个品种转基因抗虫棉在蕾期、花铃期均与对照常规棉差异显著;速效磷含量在苗期最低,吐絮期最高,其中鲁棉研28号在蕾期显著高于对照常规棉10.29%,鲁棉研36号在苗期显著高于对照常规棉6.77%。研究表明,转基因抗虫棉的种植和生育期是影响棉花根际 Bt 蛋白残留量、速效养分含量的主要因子。
Here we have detected residues of Bt protein in the rhizosphere soil of four transgenic Bt cottons and non-Bt cottons varieties at different growth stages with ELISA kit, and the changes of available nutrient content were compared at the same time.Results showed that:(1) Bt protein content reached the maximum in squaring stage.In the whole growth period, the Bt protein content in rhizosphere soil of four varieties of transgenic cottons were significantly higher than no-Bt cotton ( P <0.05).The Bt protein content of Lumian No.28 was significantly higher than that in the conventional cotton 1 029.66% in squaring stage, the protein of Lumian No.36 was significantly higher than that in the conventional cotton 2 573.45% in squaring stage, Shannong No.8 was significantly higher than conventional cotton 1 028.89% in seeding stage and Xinqiu No.4 was significantly higher than conventional cotton 793.18% in flowering and boll stage.(2) The content of nitrate nitrogen reached the maximum in seeding stage, minimum in boll-opening stage, and transgenic Bt cottons were significantly different from their conventional cottons in many stages.The content of ammonium nitrogen reached the maximum in seeding stage, minimum in boll-opening stage.The four varieties of transgenic cottons were significantly different from their conventional cottons in squaring stage, flowering and boll stage.The contents of available phosphorus reached the minimum in seeding stage, maximum in boll-opening stage.Lumian No.28 was significantly higher than conventional cotton 10.29% in squaring stage, and Lumian No.36 was significantly higher than conventional cotton 6.77% at seeding stage.Results showed that planting and growth period of transgenic Bt cotton were the main factors affecting the residue of Bt protein and the content of available nutrients in the rhizosphere.
作者
陈彦君
任梦云
关潇
朱文娟
沈奇
CHEN Yan-jun;REN Meng-yun;GUAN Xiao;ZHU Wen-juan;SHEN Qi(Chinese Research Academy of Environmental Sciences,Beijing 100012,China;Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China;Institute of Crops and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021,China)
出处
《干旱地区农业研究》
CSCD
北大核心
2019年第3期123-128,共6页
Agricultural Research in the Arid Areas
基金
环保公益性行业科研专项(201509044)
