摘要
CPP(cysteine-rich polycomb-like protein)转录因子广泛存在于动植物中,是一类成员数目较少的转录因子家族,在调控植物生长发育和响应非生物胁迫中起重要作用。该研究通过对茶树基因组系统的鉴定,获得10个CsCPP转录因子均具有典型的CXC结构域。系统发育分析将CsCPP家族成员分为4类(A^D),且大部分成员与葡萄在进化关系上更为接近。A类和C类成员的CXC结构域分布在蛋白序列的N端,而B类和D类成员分布在C端。茶树组织表达分析表明,CsCPP转录因子在生长活跃的顶芽和嫩叶中普遍高表达,不同组织的表达水平排序为:顶芽和嫩叶>根和茎>成熟叶和果>老叶和花。启动子分析发现了CsCPP家族成员的启动子区域存在大量的ABA和干旱响应元件;干旱处理下,6个CsCPP成员的表达水平均有不同程度上调,其中4个成员在ABA处理后表达迅速上调,表明CsCPP转录因子可能在ABA介导的干旱胁迫响应中起正调控作用;低温处理下,大部分CsCPP成员的表达均有轻微下调,而CsCPP 2和CsCPP 6的表达水平在6 h达到顶峰,表达量均超过2倍。研究结果为进一步发掘茶树CPP转录因子家族的功能奠定了基础。
The CPP(cysteine-rich polycomb-like protein)transcription factor family has a small number of members and is widely distributed in plants and animals.It plays an important role in regulating plant growth and development and responding to abiotic stress.In this study,10 CsCPP transcription factors were obtained by systematic identification of the tea plant genome,all of which have typical CXC domains.The phylogenetic analysis divided the CsCPP family members into four categories(A-D),and most of the members were closer to the evolutionary relationship of the grapes.The CXC domains of class A and class C members were distributed at the N-terminus of the protein sequence,while the class B and class D members were distributed at the C-terminus.The expression analysis of tea plant tissues indicated that CsCPP transcription factors were highly expressed in the growing active apical buds and young leaves.The order of expression of different tissues was mainly:apical buds and young leaves>roots and stems>mature leaves and fruits>old leaves and flowers.Promoter analysis revealed a large number of ABA and drought response elements in the promoter region of the CsCPP family members.The expression levels of six CsCPP members under drought treatment increased to varying degrees.Four of them were up-regulated after ABA treatment,suggesting that CsCPP transcription factor may play a positive role in ABA-dependent drought stress response.The expression of most CsCPP members was slightly down-regulated under low temperature treatment,while the CsCPP 2 and CsCPP 6 reached more than 2-fold at 6 h.These results laid the foundation for further exploration of the function of tea plant CPP transcription factor family.
作者
杨如兴
王鹏杰
陈芝芝
张磊
叶乃兴
尤志明
YANG Ruxing;WANG Pengjie;CHEN Zhizhi;ZHANG Lei;YE Naixing;YOU Zhiming(Tea Research Institute,Fujian Academy of Agricultural Sciences,Fu an,Fujian 355015,China;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2019年第6期1024-1032,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家茶叶产业技术体系项目(CARS-19)
福建省属公益类科研院所基本科研专项(2017R1012-2)
福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项(闽教科[2015]75号)
关键词
茶树
CPP转录因子
表达分析
胁迫
Camellia sinensis
CPP transcription factors
expression analysis
stress
