摘要
目的分析支气管哮喘患者血清中白细胞介素-33(IL-33)及1-磷酸鞘氨醇(S1P)的水平及临床意义。方法选择2015年9月~2017年5月汕头大学医学院第二附属医院(以下简称“我院”)就诊的83例支气管哮喘患者,根据喘息控制情况分为急性期组(n=48)和缓解组(n=35);选取我院同期40名健康体检者为对照组。采用酶联免疫吸附法(ELISA)检测血清中IL-33及S1P水平。测定肺功能指标:第一秒用力呼气量(FEV1)、最大呼气峰流速值(PEF)以及第一秒用力呼气量/用力肺活量(FEV1/FVC)值。应用Pearson相关系数分析患者血清IL-33及S1P水平与肺功能指标的相关性。结果急性期组及缓解组患者的血清IL-33、S1P水平均高于对照组,急性期组患者亦高于缓解组患者,差异有统计学意义(均P <0.05);急性期组及缓解组患者的肺功能指标FEV1、PEF以及FEV1/FVC均低于对照组,而急性期组患者亦低于缓解组患者,差异有统计学意义(均P <0.05)。Pearson相关性分析发现,急性期组及缓解组患者的血清IL-33及S1P水平与肺功能指标均呈负相关(r <0,P <0.05)。结论支气管哮喘患者血清IL-33、S1P水平显著上升,与肺功能呈负相关,检测血清IL-33、S1P水平有助于支气管哮喘的病情判定。
Objective To analyze the levels of interleukin 33 (IL-33) and 1- phosphosphingosine (S1P) in serum of patients with bronchial asthma and their clinical significance. Methods From September 2015 to May 2017, 83 patients with bronchial asthma admitted to the Second Affiliated Hospital of Shantou University School of Medicine ("our hospital" for short) were selected and divided into acute stage group (n = 48) and relief group (n = 35) according to the control of wheeze, 40 healthy subjects in our hospital in the same period were selected as the control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of IL-33 and S1P in serum. Lung function measures, forced expiratory volume in the first second (FEV1), maximum peak expiratory flow rate (PEF), and forced expiratory volume/forced vital capacity in the first second (FEV1/FVC). Pearson correlation coefficient was used to analyze the correlation between serum IL-33 and S1P and pulmonary function indexes. Results Serum IL-33 and S1P levels in the acute stage group and the relief group were higher than those in the control group, and the levels in the acute stage group were also higher than those in the relief group, with statistically significant differences (all P < 0.05). Lung function indexes FEV1, PEF and FEV1/FVC in the acute stage group and the relief group were all lower than those in the control group, while those in the acute stage group were also lower than those in the relief group, with statistically significant differences (all P < 0.05). Pearson correlief coefficient analysis results showed that the serum IL-33 and S1P levels in the acute phase group and the remission group were negatively correlated with lung function indicators (r < 0, P < 0.05). Conclusion Serum IL-33 and S1P levels are significantly increased in patients with bronchial asthma, which is negatively correlated with lung function. The detection of serum IL-33 and S1P levels is helpful for the diagnosis of bronchial asthma.
作者
许惠娟
宋梅
林钊钒
XU Huijuan;SONG Mei;LIN Zhaofan(Respiratory Department, the Second Affiliated Hospital of Shantou University Medical College, Guangdong Province, Shantou 515000, China)
出处
《中国医药导报》
CAS
2019年第16期87-90,共4页
China Medical Herald
基金
广东省基础与应用基础研究专项(广东省自然科学基金项目)(2014A030313105)
广东省汕头市医疗科技计划项目(20150406)
