摘要
为了研究新会陈皮的降糖活性,首先制备了5年和10年陈皮的乙醇提取物,再使用5种不同极性溶剂进行萃取,分别得到石油醚层、二氯甲烷层、乙酸乙酯层、正丁醇层以及水层提取物。接着评估这些粗提物对α-葡萄糖苷酶的体外抑制活性。结果表明:5年和10年陈皮提取物的石油醚层对α-葡萄糖苷酶的IC50均为0.2mg/mL,二氯甲烷层的IC50分别为8.3、8.7mg/mL,乙酸乙酯层的IC50分别为17.8、8.4mg/mL,正丁醇层和水层无明显活性,阳性对照品阿卡波糖的IC50为58.8mg/mL。本研究表明,新会陈皮具有较好的α-葡萄糖苷酶体外抑制活性,具有开发成降糖功能食品的潜力,本研究为新会陈皮的深度开发提供了科学依据和参考。
In order to study the hypoglycemic activity of Xinhui Chenpi,firstly,ethanol extract of 5-years-old and 10-years-old Chenpi were prepared. Then the ethanol extract was further extracted by 5 solvents with different polarity,giving petroleum ether,dichloromethane,ethyl acetate,n-butyl alcohol and water layers. Then,the inhibition of α-glucosidase of these extracts in vitro were evaluated. Results show that both IC50 values of petroleum ether layers of 5-years-old and 10-years-old Chenpi are 0.2 mg/mL. The IC50 values of dichloromethane layer are 8.3 and 8.7 mg/mL,respectively. The IC50 values of ethyl acetate layer are 17.8 and 8.4 mg/mL,respectively. No obvious inhibition activity of n-butyl alcohol and water layers were observed. The IC50 of positive reference acarbose is 58.8 mg/mL. The results suggest that Xinhui Chenpi possesses good inhibition activity of α-glucosidase and has the potential to develop into hypoglycemic functional food. This study provide scientific basis and reference for the deep development of Xinhui Chenpi.
作者
谭乾开
李裕琳
唐小文
Tan Qiankai;Li Yulin;Tang Xiaowen(School of Biotechnology and Health Science,Wuyi University,Jiangmen 529020,Guangdong,China;Enping Agricultural Bureau,Enping 529400,Guangdong,China)
出处
《云南化工》
CAS
2019年第5期63-65,共3页
Yunnan Chemical Technology
基金
广东省大学生创新创业训练计划项目
关键词
陈皮
Α-葡萄糖苷酶
提取物
降糖
Chenpi
α-glucosidase activity
extract
hypoglycemic
