摘要
以贵州禾来拢茎尖为材料,遗传转化含有Ubiqutin启动子的苦瓜几丁质酶基因McCHIT1,设计正交试验,探究农杆菌菌液浓度、真空渗透压和真空处理时间对贵州禾来拢茎尖遗传转化的影响。结果表明:对植株的死亡率及转化率影响最大的是真空处理时间,其次为菌液浓度,真空渗透压的影响较小。综合考虑植株死亡率及转化效率认为,菌液侵染浓度OD6000.8,真空处理时间11min,真空渗透压16kPa为农杆菌介导贵州禾来拢茎尖遗传转化的最优参数。
In the study,we used the Guizhou He Lailong’s shoot tips as the research material to transform McCHIT1 containing the Ubiqutin promoter,and investigated the influence of the concentration of agrobacterium culture solution,vacuum osmotic pressure and vacuum treatment time on the efficiency of agrobacterium-mediated genetic transformation of Guizhou He Lailong’s shoot tips by orthogonal experimental design method.The results showed that the vacuum treatment time had the greatest influence on plant mortality and conversion rate,followed by the concentration of bacteria solution,and the vacuum osmotic pressure had little influence.According to the comprehensive consideration of plant mortality and transformation efficiency,the optimal parameters for agrobacteria-mediated genetic transformation of the shoot tip of Guizhou He Lailong were the infection concentration OD 600 0.8,vacuum treatment time 11 min and vacuum osmotic pressure 16 kPa.
作者
刘怒安
曾晓芳
赵德刚
LIU Nuan;ZENG Xiaofang;ZHAO Degang(Institute of College of Life Sciences and Agro-Bioengineering,Guizhou University/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education),Guizhou Key Lab of Agro-Biological Engineering,Guiyang Guizhou 550025,China;DUS Center,National Department of Agriculture and Villages,Guizhou Academy of Agricultural Sciences,Guiyang Branch,Guiyang Guizhou 550006,China)
出处
《种子》
北大核心
2019年第6期25-29,共5页
Seed
基金
国家转基因新生物品种培育重大专项(2016ZX08010003-009)
贵州省科技合作计划项目(黔科合LH字[2016]7448号)
贵州省科技计划项目(黔科合基础[2017]1039)
贵州省高层次创新新人才培养项目(黔科合人才(2016)4003号)
关键词
水稻
遗传转化
来拢
rice
genetic transformation
Lailong
