摘要
本研究通过对化学诱变剂甲基磺酸乙酯(Ethyl methyl sulfone,EMS)处理贵州地方水稻品种来拢所构建的突变体库进行筛选,得到一份能稳定遗传的大粒突变体bs-1。对该突变体进行形态学和细胞学观察、光合生理特性研究及稻米品质分析,结果表明:与野生型(wild type,WT)相比,bs-1突变体表现出节间数增加一节,各节间长度缩短,节间细胞增长,谷粒长和谷粒宽增大。野生型和突变体在生长发育的前中期光合速率均逐渐增加,但各时期bs-1突变体净光合速率较野生型高。bs-1突变体的籽粒16种氨基酸、蛋白质、粗纤维及微量元素铁含量减少,直链淀粉、苯丙氨酸和微量元素硒含量增加,其胶稠度、碱消值、微量元素锌含量与野生型差异不明显。
A big seed mutant bs-1 was obtained and identified from EMS （Ethyl methyl sulfone） treated Guizhou native rice variety Lailong. The morphological and cytological observation, as well as photosynthetic characteristics and quality evaluation of this mutant have been investigated and analyzed in this study. The results showed that bs-1 mutant has the phenotype of one more internode, shorten internode length, longer internode cell and larger grain size compared to wild type. Both the bs-1 mutant and wild type maintain a increasing photosynthetic rate during the early stage of growth, while a higher net photosynthetic rate was observed in bs-1 plants during all growth stages. Comparison of rice quality traits between bs-1 mutant and wild type indicated that bs-1 mutant has the same level of gel consistency, alkali spreading value, zinc content as wild type. While it has lower content of 16 amino acids, crude protein, crude fiber and iron and higher content of amylase, phenylalanine and selenium.
出处
《种子》
CSCD
北大核心
2014年第7期5-9,14,共6页
Seed
基金
国家转基因生物新品种培育科技重大专项"安全转基因技术研究"(编号:2013 zx08010-003)
贵州省科技重大专项"贵州特有水稻种质资源优异基因克隆及育种技术研究应用"(编号:黔科合重大专项字[2012]6005号)
关键词
贵州地方稻种来拢
bs-1突变体
光合特性
品质性状
guizhou native rice variety Laitong
bs-1 mutant
photosynthetic characteristics
quality traits
