摘要
目的探讨ACE2-Ang(1-7)-MasR轴在大鼠心脏骤停-心肺复苏-自主循环恢复后(CA-CPRROSC)心、肝、肾功能损伤中的作用,为提高ROSC后患者生存率提供实验依据。方法建立CA-CPR模型,随机将大鼠分为假手术组、常规复苏组、Ang(1-7)组及A779组,监测各组血流动力学指标、生化指标、心肌病理性损伤情况。结果与假手术组比较,其余3组心率(HR)、平均动脉压(MAP)、±LVdp/dtmax下降(P <0.05),ROSC后6 h的BNP、cTnT、Cr、ALT升高(P <0.05)。与常规复苏组相比,Ang(1-7)组各时点±LVdp/dtmax增高,ROSC后6 h的BNP、cTnT均降低(P <0.05),而A779组则相反。结论大鼠在CA-CPRROSC后的心、肝、肾功能出现一定程度的损害,促进该轴活化参与大鼠心肺复苏后器官功能的保护作用。
Objective To explore the role of ACE2-Ang(1-7)-MasR axis in the injury of heart,liver and kidney function after cardiac arrest-cardiopulmonary resuscitation-autonomic circulation recovery(CA-CPR-ROSC)in rats,and to provide experimental basis for improving the survival rate of patients with ROSC. Methods Rats were randomly divided into sham-operated group,conventional resuscitation group,MasR agonist Ang(1-7)group and MasR antagonist A779 group,18 rats in each group. The CA-CPR model was established to monitor the hemodynamic,biochemical and pathological changes of myocardium in each group. Results Compared with shamoperated group,HR,MAP,+LVdp/dtmax were decreased in the other three groups(P < 0.05),while BNP,cTnT,Cr and ALT were increased 6 hours after ROSC(P < 0.05). Compared with routine resuscitation group,Ang(1-7)group had higher(+LVdp/dtmax)at all time points and lower BNP and cTnT at 6 hours after ROSC(P < 0.05),while A779 group had the opposite effect. Conclusion Cardiac function,liver and kidney function are impaired after CA-CPR-ROSC in rats,and ACE2-Ang(1-7)-MasR axis activation is involved in the protection of organ function after cardiopulmonary resuscitation.
作者
袁琦松
朱丽
王炳今
李岚
黄佳
周霞
周厚荣
YUAN Qisong;ZHU Li;WANG Bingjin;LI Lan;HUANG Jia;ZHOU Xia;ZHOU Hourong(Department of General Medicine,Guizhou People′s Hospital,Guiyang 550002,China)
出处
《实用医学杂志》
CAS
北大核心
2019年第12期1915-1919,共5页
The Journal of Practical Medicine
基金
2016年北京协和医学基金会专项基金项目(编号:RE2016-005)
2016年贵州省科学技术基金重点项目(编号:黔科合基础[2016]1411)
