摘要
目的观察窒息大鼠心肺复苏术后血清肾素、血管紧张素Ⅱ(AngⅡ)以及心肌肾素、AT1、AT2mRNA的表达情况。方法随机将42只SD大鼠分为正常对照组(A组)、假手术组(B组)、CPR组(C组)、CPR+肾上腺素组(D组)四组,B、C、D三组均分为0h、6h两个亚组。A组未行有创操作,采血后处死取心肌组织;B、C、D三组均做动静脉穿刺,B组予普通上氧(3L/min),C组及D组给予气管切开、有创机械通气及心肺复苏。B、C、D三组于各亚组时间终点采血测血清肾素、AngⅡ表达水平,处死动物后留心肌标本检测心肌肾素、AT1R、AT2R的mRNA表达。B、C、D三组监测平均动脉压(MAP)、左室收缩峰压(LVSP)、左室舒张末期压力(LVEDP)、左室最大上升与下降速率(±LVdP/dtmax)。结果 (1)心肺复苏术后6h,血清肾素及血管紧张素Ⅱ水平以及心肌肾素、AT1、AT2mRNA表达上调,与A组比较差异有统计学意义(P<0.01);(2)复苏后6h,C组与B组比较,血清肾素、血管紧张素Ⅱ表达水平明显增高(P<0.05),但心肌肾素、AT1、AT2mRNA差异无统计学意义(P>0.05);(3)相关性分析结果提示,AngⅡ及AT1R mRNA与±dp/dtmax、LVSP呈正相关,与LVEDP呈负相关,差异均有统计学意义(P<0.01)。结论心肺复苏可引起全身及心肌局部RAS系统激活;肾上腺素可促使血清肾素、血管紧张素Ⅱ及心肌肾素、AT1R、AT2R mRNA呈现过表达;ACE-AngⅡ-AT1轴的激活是复苏后心功能不全原因之一。
Objective To observe the serum renin,angiotensin II（AngⅡ）and mRNA expression of myocardial renin,AT1 and AT2after cardiopulmonary resuscitation（CPR）.Method 42 SD rats were randomly divided into 4groups which were normal control group（group A,n=6）,the sham-operation group（group B,n=12）,CRP group（group C,n=12）,and CPR ＋ adrenaline group（group D,n=12）.Group B,C and D were all subdivided into subgroup 0h（n=6）and subgroup 6h（n=6）.Group A was not given invasive operation.The rats were taken for blood and scarified for myocardial tissue.Group B,C and D were given artery and vein puncture.Group B was given ordinary oxygen（3L/min）.Group C and group D were given tracheotomy,invasive mechanical ventilation and CPR.Group B,C and D were taken blood for serum renin and AngⅡ at terminal time point of each subgroup.The animals were sacrificed for myocardial specimens to test mRNA expression of myocardial renin,AT1 Rand AT2R.Group B,C and D were monitored for mean arterial pressure（MAP）,left ventricular systolic pressure（LVSP）,left ventricular end-diastolic pressure（LVEDP）and left ventricular maximum rising and falling rate（±LVdP/dtmax）.Result 6hafter CPR,expression of serum renin,AngⅡ,and mRNA expression of cardiac renin,AT1 and AT2were increased,which was of statistical significance compared to group A（P〈0.01）.6hafter CPR,compared to group B,serum renin and AngⅡ were obviously increased in group C,with statistical significance（P〈0.05）.But the mRNA expression of myocardial renin,AT1 and AT2were slightly increased,but there were no statistical significance（P〉0.05）.Correlation analysis was shown that mRNA of AngⅡ and AT1 R was positively correlated to ±dp/dtmax and LVSP,and negatively correlated to LVSP,and there were statistical significantly differences（P〈0.01）.Conclusion CPR can active systemic and myocardial RAS system.Adrenaline makes over-expression of serum renin,angiotensinⅡand mRNA of cardiac renin,AT1 Rand AT2R.Activation of ACE-A
出处
《贵州医药》
CAS
2015年第8期677-681,共5页
Guizhou Medical Journal
基金
贵州省科技计划基金资助课题[黔科合LS字(2012)014号]
贵州卫生计生委计划基金资助项目[gzwjwj(2014-1-049)]
关键词
AngⅡ
心功能不全
心肺复苏
AngⅡ
Cardiac infufficiency
Cardiopulmonary resuscitation（CPR）
