摘要
目的建立兔脊柱旁肌肉VX2种植瘤模型,观察其生物学特性。方法CT导引下经皮穿刺将VX2肿瘤组织块种植于15只新西兰大白兔脊柱旁肌肉,构建肿瘤模型。种植术后第14、21、28、35天行CT平扫和增强扫描,随机选取1只荷瘤兔处死,作大体解剖及病理学检查。结果15只实验兔脊柱旁肌肉VX2肿瘤组织块均成功种植,建模成功14只,成瘤率为93.3%。种植瘤CT平扫呈低密度结节灶,边缘模糊;CT增强扫描病变呈环状强化,边缘模糊,中心见低密度坏死区。种植瘤生长迅速,第28天与第21天肿瘤生长率(TGR)比较,差异有显著统计学意义(P=0.004);第35天与第28天TGR比较,差异有显著统计学意义(P=0.01)。种植术后第14~28天,瘤体大小为1.0~2.5 cm。种植瘤坏死率第14、21、28天间差异均有显著统计学意义(P<0.01),第28、35天间差异无统计学意义(P=0.243)。种植瘤病理组织学呈浸润性生长,内部粥糜样坏死;肿瘤细胞呈条索状或巢状弥漫分布,间质血管丰富;肿瘤细胞形态不规则,体积较大,细胞异型性明显并见较多核分裂像。第35天时,5只荷瘤兔CT增强扫描发现腹膜后淋巴结和肺转移,2只荷瘤兔脊髓受侵犯。结论脊柱旁肌肉种植瘤恶性度高,生长速度快,成瘤时间短,瘤组织血供丰富,是一种较为理想的用于肿瘤局部治疗研究的实验模型。
Objective To establish rabbit VX2 tumor model in paraspinal muscle and to observe its biological characteristics.Methods Through CT-guided percutaneous puncture,VX2 tumor tissue block was implanted into the paraspinal muscle in 15 New Zealand white rabbits to establish tumor models.On the14 th,21 st,28 th and 35 th day after inoculation,both plain CT scan and contrast-enhanced CT scan were separately performed,and one tumor-bearing rabbit was randomly selected and sacrificed for gross anatomy and pathological examination.Results Successful implantation of VX2 tumor tissue block into the paraspinal muscle was accomplished in all 15 experimental rabbits,successful modeling was obtained in 14 rabbits,and tumor formation rate was 93.3%.On plain CT scan,the implanted tumor presented as a low-density nodular focus with blurred edges.On contrast-enhanced scan the lesion showed ring-shaped enhancement with blurred border and low-density shadow of central necrotic area.The implanted tumors grew rapidly.The tumor growth rate(TGR)at the 28 th day was statistically significant different from that at the 21 st day(P=0.004).Statistically significant difference in TGR existed between the value determined at the 35 th day and the value determined at the 28 th day(P=0.01).During 14-28 day after inoculation,the tumor size was 1.0-2.5 cm.Statistically significant difference in necrosis rate of implanted tumor existed between each other among the values determined at the 14 th,21 st and 28 th day(P<0.01),but the difference in the necrosis rate between the values determined at the 28 th day and 35 th day was not statistically significant(P=0.243).Pathologically,the implanted tumor exhibited infiltrating growth pattern with central porridge-like erosion necrosis,the tumor cells diffusely distributed in the form of strips or nests with abundant interstitial vessels.The tumor cells were irregular in shape and large in volume with obvious cell heteromorphism and nuclear fission picture.On the35 th day,enhanced CT scan revealed retroperit
作者
王东东
彭金钊
李云芳
李晓光
WANG Dongdong;PENG Jinzhao;LI Yunfang;LI Xiaoguang(Peking Union Medical College & ChineseAcademy of Medical Sciences,Center of Minimally - Invasive Therapies for Tumors of Beijing Hospital,National Center of Gerontology y Beijing 100061,China)
出处
《介入放射学杂志》
CSCD
北大核心
2019年第6期561-565,共5页
Journal of Interventional Radiology
关键词
VX2
脊柱旁肌肉
动物模型
CT
VX2
paraspinal muscle
animal model
computed tomography
