摘要
【目的】为了提高胞外多糖的产量,优化柱状田头菇液体发酵适宜的培养基和培养条件。【方法】以胞外多糖产量为主要测定指标,通过对培养基的碳源、氮源种类及其浓度、起始pH、摇床转速、培养时间等发酵条件进行单因素试验,以获得柱状田头菇液体深层发酵的优化条件,提高柱状田头菇菌丝产胞外多糖的产量,其中胞外多糖产量通过苯酚-硫酸法测得。【结果】柱状田头菇液体发酵产胞外多糖的优化条件为:采用可溶性淀粉作为碳源,浓度控制在20~40 g/L;采用L-谷氨酰胺作为氮源,浓度控制在2 g/L;起始pH控制在7.0,转速控制在180 r/min,并在26℃下培养12 d。【结论】在此发酵条件下,柱状田头菇液体发酵的胞外多糖产量可达到1.08 g/L,其中多糖含量为71.73%、蛋白质含量为8.62%,可为柱状田头菇多糖的工业化生产和应用提供理论依据。
【Objective】In order to improve the production of extracellular polysaccharides of Agrocybe aegerita, the suitable medium and related culture conditions for liquid fermentation were optimized.【Method】Based on the yield of extracellular polysaccharides, some fermentation conditions such as carbon source, nitrogen source and concentration, initial pH, shaking speed and culture time were explored by single factor test. The optimized conditions of submerged fermentation increased the yield of extracellular polysaccharide produced by the mycelium of A. aegerita, and the yield of extracellular polysaccharide was measured by the phenol-sulfuric acid method.【Result】The optimum conditions for the production of extracellular polysaccharides by liquid fermentation of A. aegerita were as follows: soluble starch was used as carbon source and the concentration was controlled at 20-40 g/L;L-glutamine was used as nitrogen source and the concentration was controlled at 2 g/L;the initial pH was controlled at 7.0, the shaking speed was controlled at 180 r/min, and extracellular polysaccharides were cultured at 26℃ for 12 d.【Conclusion】Under the above-mentioned fermentation conditions, the yield of extracellular polysaccharide was 1.08 g/L, in which the content of polysaccharide was 71.73% and the content of protein was 8.62%. This provided an theoretical basis for the industrial production and application of A. aegerita polysaccharides.
作者
郑晓雅
殷伟伟
张松
兰瑛
ZHENG Xiaoya;YIN Weiwei;ZHANG Song;LAN Ying(Life of Sciences, South China Normal University, Guangzhou 510631, China;Shenzhen Huishangke Technology Co., Ltd, Shenzhen 518055, China)
出处
《广东农业科学》
CAS
2019年第5期25-32,共8页
Guangdong Agricultural Sciences
基金
广东省科技计划项目(2016A010105019)
广州市科技计划项目(201707010138)
深圳市科技计划项目(CYZZ20160526154742735)
关键词
液体发酵
条件优化
多糖产量
碳源
氮源
苯酚-硫酸法
liquid fermentation
condition optimization
polysaccharide yield
carbon source
nitrogen source
phenolsulfuric acid method
