摘要
以从泡菜中筛选出的1株亚硝酸盐降解能力较高的植物乳杆菌5-7-3为研究对象,对其培养条件进行优化,提高亚硝酸盐的降解率。研究培养温度、发酵液起始pH、接种龄、接种量这些单因素对植物乳杆菌5-7-3降解亚硝酸盐效果的影响,并利用Box-Behnken中心组合试验设计原理设计植物乳杆菌5-7-3培养条件模型,得到亚硝酸盐降解率最高的培养条件:温度37℃,pH 6,接种量8%,接种龄21h。该培养条件下菌株的亚硝酸盐降解率达到89.94%,比优化前提高了4.98%。
Lactobacillus plantarum 5-7-3 with high nitrite degradation ability is selected from kimchi,and its culture conditions are optimized to improve the nitrite degradation rate.The effects of single factors such as culture temperature,pH value of fermentation broth,inoculation age and inoculation amount on nitrite degradation effect by L.plantarum 5-7-3 are studied.Based on this result,the culture condition model of L.plantarum 5-7-3 is designed by Box-Behnken central experimental design principle,and the highest degradation rate of nitrite is obtained under the conditions of 37℃,pH 6,inoculation amount 8%and inoculation age 21 h.Under these conditions,the degradation rate of nitrite is 89.94%,which is 4.98%higher than that before optimization.
作者
杨晶
黄玉军
陈大卫
关成冉
姚瑶
印伯星
杨仁琴
YANG Jing;HUANG Yu-jun;CHEN Da-wei;GUAN Cheng-ran;YAO Yao;YIN Bo-xing(Key Lab of Dairy Biological Technology and Safety Controlin Jiangsu Province,Yangzhou University,Yangzhou 225009,China;Jiangsu Provincial Dairy Biotechnology Research Center,Yangzhou 225009,China)
出处
《中国调味品》
CAS
北大核心
2019年第4期69-74,共6页
China Condiment
基金
江苏省高校自然科学研究重大项目(17KJA550004)
国家自然科学基金青年基金项目(31700079)
国家自然科学基金青年基金项目(31701627)
江苏省高校自然科学研究面上项目(17KJB550009)
关键词
植物乳杆菌
亚硝酸盐
降解
培养条件
优化
Lactobacillus plantarum
nitrite
degradation
culture conditions
optimization
