摘要
转录因子Nrf2对模式生物抗氧化酶的调控起关键作用,但迄今为止尚未见Nrf2具有对害螨抗氧化酶调控功能相关报道。本研究将模式生物研究中已知的Nrf2基因抑制剂、激活剂处理朱砂叶螨,通过抑制、激活其转录因子TcNrf2的表达,研究其对下游调控的抗氧化酶基因TcSOD和TcCAT表达的影响。结果表明,采用抑制剂护鸦胆子苦醇处理时,朱砂叶螨取食抗、感木薯品种1、4、8d后,TcNrf2、TcSOD和TcCAT的基因表达量均显著降低,而采用抑制剂视黄酸处理时上述基因表达不受影响;采用激活剂特丁基对苯二酚和二噻环戊二烯硫酮分别处理后,朱砂叶螨TcNrf2、TcSOD和TcCAT的基因表达量均显著提高,说明TcNrf2及其调控的TcSOD和TcCAT基因表达量变化趋势具有一致性。本研究从分子水平直接证明TcNrf2基因在木薯抗螨中具有重要作用,为木薯抗螨分子机理研究奠定理论基础。
The transcription factor Nrf2 plays a key role in the regulation of antioxidant enzymes in model organisms,but up to now, there was a lack of study indicating that Nrf2 can regulate antioxidant enzymes in mites. In this study, the Nrf2 inhibitors and activators were used to treat with the red spider mite(Tetranychus cinnabarinus). The transcription of TcSOD and TcCAT were studied by inhibiting and activating the transcription factor TcNrf2. The results showed that the transcriptions of TcNrf2, TcSOD and TcCAT decreased significantly after feeding on mite-resistant and mite-susceptible cassava cultivars for 1, 4 and 8 d, while were not affected by retinoic acid treatment. However, the transcription of those genes significantly increased after treated by TBHQ(tert-butylhydroquinone) and D3T(1,3-dithiole-2-thione-4,5-dithiolat), respectively, suggesting that the transcriptions of TcNrf2 and its regulated TcSOD and TcCAT genes were consistent. This study directly proves that TcNrf2 gene plays an important role in cassava mite resistance at the molecular level, which would lay a theoretical foundation for the molecular mechanism of mite resistance to cassava.
作者
高锦涛
梁晓
伍春玲
陈青
陈谦
Gao Jintao;LIANG Xiao;WU Chunling;CHEN Qing;CHEN Qian(Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China)
出处
《热带作物学报》
CSCD
北大核心
2019年第6期1163-1170,共8页
Chinese Journal of Tropical Crops
基金
国家木薯产业技术体系虫害防控岗位科学专项(No.CARS-11-hncq)
国家自然科学基金青年基金项目(No.31601649)
农业部橡胶树生物学与遗传资源利用重点实验室开放课题重点基金项目(No.RRI-KLOF201602)
关键词
木薯
朱砂叶螨
转录因子TcNrf2
调控功能
cassava
Tetranychus cinnabarinus
transcription factor TcNrf2
regulation function
