摘要
采用微波辐照对木聚糖酶产生菌疏棉状嗜热霉菌(Thermomyces lanuginosus)CICC2691进行诱变选育,考察不同微波功率和辐照时间对菌株致死率和正突变率的影响,得到最佳的诱变条件:微波辐照功率为500W,辐照时间为60s。在最佳诱变条件下得到一株高产木聚糖酶且能稳定遗传的菌株W5-133,其所产木聚糖酶酶活较出发菌株提高了66.8%。以菌株W5-133为研究对象,经单因素试验和响应面分析法对其发酵产酶条件进行了优化,得到最优发酵产酶条件:葡萄糖质量浓度为2.35g/L,发酵温度为56.0℃,发酵时间为99.5h,初始pH值为5.8,该条件下的木聚糖酶酶活为186.81U/mL,比优化前提高了24.4%。
Microwave irradiation mutagenesis was used to mutagenize xylanase-producing strain of Thermomyces lanuginosus CICC 2691. Optimum conditions of 500 W of the microwave irradiation power and 60 s of the irradiation time were investigated and obtained according to the lethality and positive mutation rate. Under optimal conditions, a strain named W5-133 with high xylanase capacity and hereditary stability was obtained. The ability of xylanase production increased by 66.8% compared to the original strain. Then, the fermentation conditions of W5-133 were optimized by single factor and response surface optimization methods. The optimal conditions were as follows: Glucose content 2.35 g/L, fermentation temperature 56.0℃, fermentation period 99.5 h, initial pH 5.8. Under these conditions, the xylanase activity of W5-133 achieved 186.81 U/mL, increased by 24.4% before optimization.
作者
朱慧霞
方桢
汪水玲
马晓静
姚日生
ZHU Hui-xia;FANG Zhen;WANG Shui-ling;MA Xiao-jing;YAO Ri-sheng(School of Food and Biological Engineering, Heifei University of Technology, Heifei 230009, China;Engineering Research Center of Bio-process from Ministry of Education, Heifei 230009, China)
出处
《纤维素科学与技术》
CAS
CSCD
2019年第2期11-23,共13页
Journal of Cellulose Science and Technology
基金
国家高技术研究发展计划(863计划)项目(SS2014AA021902)
国家自然科学基金青年基金项目(31400049)
安徽省教育厅自然科学研究项目重点项目(非产学研)(2013AJZR0044)
关键词
疏棉状嗜热霉菌
微波诱变
木聚糖酶
响应面分析法
发酵条件优化
Thermomyces lanuginosus
microwave mutagenesis
Xylanase
response surface analysis
optimization of fermentation condition
