摘要
以米曲霉KA2308作为出发菌株,首次利用常压室温等离子体诱变技术选育高产曲酸菌株,通过96孔板高通量筛选获得一株曲酸生产菌KA1515,曲酸产量提高到47.28g·L-1,比出发菌株提高了31.48%,经过多次传代,曲酸产量仍比较稳定。表明采用常压室温等离子体诱变能有效提高米曲霉曲酸产量。
The strain of Aspergillus oryzae KA2308 was exposed on atmospheric and room temperature plasma（ARTP）jet to improve kojic acid production for the first time.A high kojic acid-producing strain KA1515 was obtained by high-throughput screening method with 96-well plates.The yield of kojic acid of mu-tant reached up to 47.28 g·L-1 ,increasing by 31.48% compared with original strain.Kojic acid yield was sta-ble after several passages.The results showed that mutation by atmospheric and room temperature plasma could raise kojic acid yield of Aspergillus oryzae effectively.
出处
《化学与生物工程》
CAS
2015年第5期52-54,60,共4页
Chemistry & Bioengineering
关键词
米曲霉
曲酸
常压室温等离子体诱变
突变株
高通量筛选
Aspergillus oryzae
kojic acid
atmospheric and room temperature plasma （ARTP）
mutantstrain
high-throughput screening
