摘要
目的探讨转化生长因子(TGF)-β1与含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶4(ADAMTS4)在人颈动脉粥样硬化(AS)斑块中表达的关系。方法收集颈动脉内膜剥脱术(CEA)后的斑块组织(n=46),以苏木精-伊红(HE)染色后依据病理学特征分为稳定斑块组和易损斑块组;分别采用免疫组织化学(IHC)与实时定量反转录聚合酶链反应(RT-qPCR)检测ADAMTS4与TGF-β1的表达部位及水平,以脐动脉作为对照(n=10)进行比较分析,应用SPSS23.0统计软件分析。结果易损斑块组、稳定斑块组及对照组的ADAMTS4mRNA表达量分别为4.43±2.02、3.16±1.57、1.02±0.22,组间比较差异有统计学意义(F=17.545,P<0.05);易损斑块组、稳定斑块组及对照组的TGF-β1mRNA表达量分别为3.57±1.76、4.68±1.42、1.14±0.31,组间比较差异有统计学意义(F=24.676,P<0.05);Pearson相关分析显示斑块中两者的表达量呈正相关(r=0.439,P<0.05)。结论在AS发生发展及斑块演变过程中,斑块局部ADAMTS4与TGF-β1的表达量呈正相关性增加趋势,其机制可能与剂量或细胞类型依赖性有关。
Objective To investigate the relationship between the expression level of transforming growth factor-β1 (TGF-β1) and a disintegrin and metalloproteinase with thrombospondin motif-4 (ADAMTS4) in human carotid atherosclerotic (AS) plaques. Methods Plaque tissues (n=46) were collected after carotid endarterectomy and divided into stable plaque group and unstable plaque group according to the histopathological features by hematoxylin and eosin (HE) staining. Immunohistochemistry (IHC) and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) were used to detect the expression of ADAMTS4 and TGF-β1 in plaques, respectively. Umbilical arteries were served as the control group (n=10) and statistics analysis were conducted as needed. Results IHC results showed that both of ADAMTS4 and TGF-β1 were expressed in all plaques;RT-qPCR results showed that the expression level of ADAMTS4 mRNA was significantly higher in unstable plaque group (4.43±2.02) compared to the stable plaque group (3.16±1.57) and the controls (1.02±0.22, F=17.545, P<0.05);TGF-β1 mRNA in stable plaque group (4.68±1.42) was also higher than that in the unstable plaques (3.57±1.76) and the controls (1.14±0.31), and the difference was statistically significant between groups (F=24.676, P<0.05). Pearson correlation analysis showed that there existed a positive correlation between indexes (r=0.439, P<0.05). Conclusion ADAMTS4 and TGF-β1 were increased together during the process of AS and plaque evolution, which may be related to dose or cell type dependence.
作者
雷彤彤
蔡申华
邢森
胡杰
田琴琴
师佩路
董红霖
Lei Tongtong;Cai Shenhua;Xing Miao;Hu Jie;Tian Qinqin;Shi Peilu;Dong Honglin(Department of Vascular Surgery,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第6期1012-1015,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(8187021553).
