摘要
目的分析我国无偿献血人群艾滋病病毒I型(HIV-1)感染者病毒亚型及env基因C2-V4区序列特征。方法针对我国13个省市献血人群筛选的115例HIV-1阳性标本,扩增HIV-1的env基因C2-V4区片段并测序;构建最大似然树(Maximum Likelihood)分析不同亚型env基因序列的进化关系;分析该基因区序列变异以及2G12、PGT135、PGT128中和表位特征。结果从115例HIV阳性标本中成功扩增C2-V4区76份,经测序分析发现HIV-1基因亚型7种,其中,亚型CRF01_AE和CRF07_BC所占比例最高,分别占46.0%(35/76)和39.5%(30/76),其他亚型CRF08_BC、B、CRF65_CPX、CRF59_01B、CRF61_BC所占比例分别为5.3%(4/76)、5.3%(4/76)、1.3%(1/76)、1.3%(1/76)、1.3%(1/76)。V3环顶端四肽存在GPGQ、GPGR、GPGK、GQGR四种类型,辅助受体以CCR5(90.8%)为主。97.1%的CRF01_AE毒株分别缺失2G12和PGT135抗体识别的相关糖基化位点,而CRF07_BC毒株100%缺失2G12抗体识别相关的糖基化位点,80%毒株未缺失PGT135抗体识别相关的糖基化位点。结论我国献血人群HIV-1感染者以CRF01_AE和CRF07_BC为主要流行毒株;各毒株亚型V3顶端四肽以GPGQ变异型为主,且主要使用CCR5为辅助受体;2G12、PGT135及PGT128中和抗体表位呈现不同程度的变异。该研究为进一步开展针对献血人群的艾滋病预防与控制提供了参考数据。
Objective To investigate the HIV-1 subtypes and the sequence characteristics of C2-V4 region of HIV-1env gene among the blood donors. Methods A molecular epidemiological survey of HIV infection was conducted in the blood donors from 13 Chinese municipalities and 115 HIV-1 infected donors were identified. The env gene was amplified and sequenced;phylogenetic analysis was conducted through Maximum Likelihood (ML) trees;the gene variation and the epitope trait of broadly monoclonal neutralizing antibodies (bmNAbs) such as 2G12, PGT135 and PGT128 were analyzed. Results A total of 76 sequences of C2-V4 region were successfully obtained from 115 HIV-1 infected donors. On basis of phylogenetic analyses, seven gene subtypes of HIV-1 were identified, and subtypes CRF01_AE and CRF07_BC had the highest percentage with 46.0%(35/76) and 39.5%(30/76), respectively. The percentage of other subtypes, including CRF08_BC,B,CRF65_CPX,CRF59_01B,CRF61_BC, was 5.3%(4/76), 5.3%(4/76), 1.3%(1/76), 1.3%(1/76), 1.3%(1/76), respectively. The crown tetrapeptide of V3 loop existed in 4 types: GPGQ, GPGR, GPGK and GQGR, and the majority of strains utilize CCR5 as co-receptor (90.8%). Over 97.1% CRF01_AE had deletions of glycosylation sites associated with both 2G12 and PGT135 recognition, and for CRF07_BC, the deletion of glycosylation sites associated with 2G12 recognition at 100%, but no deletion with PGT135 at 80%. Conclusion CRF01_AE and CRF07_BC are the main strains in the HIV-1 infected blood donors;and GPGQ are recognized as the dominant type in the crown tetrapeptide of V3 loop for all strains, which mainly uses CCR5 as co-receptor. Varying degrees of epitope variation among bmNAbs such as 2G12, PGT135 and PGT128 exist in CRF01_AE and CRF07_BC strains. The research provides the reference data for further developing the study strategies on AIDS prevention and control of the blood donation population.
作者
高占
胡园园
王瑞
赵帅
王露楠
洪坤学
邢辉
葛红卫
GAO Zhan;HU Yuanyuan;WANG Rui;ZHAO Shuai;WANG Lu’nan;HONG Kunxue;XING Hui;GE Hongwei(Beijing Red Cross Blood Center, Beijing 100088, China)
出处
《中国热带医学》
CAS
2019年第6期530-534,共5页
China Tropical Medicine
关键词
人类免疫缺陷病毒
亚型
变异
无偿献血
中和抗性
HIV-1
subtype
variation
volunteer blood donors
neutralization resistance
